Analysis of vacuole formation by small organic molecules

有机小分子空泡形成的分析

• 批准号: 20611007
• 负责人: KAWAZOE Yoshinori
• 金额: $ 2.91万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20611007/
• 关键词: オルガネラ形成; 液胞; 小分子有機化合物; ケミカルバイオロジー; オルガネラ; オートファジー; オートファジー関連遺伝子; 膜融合; エンドソーム

I discovered a small organic molecule that induces a huge vacuoles in mammalian cells. The aim of this study is elucidating a molecular mechanism of vacuolation by means of this compound as a tool. To examine the vacuole origin, I conducted a immuno-fluorescense microscopic study and found that localizations of AAE1 and M6PR, early and late endosome marker protein, respectively, was different from those under normal state. And I found that endosomes are enlarged by fusion each other. Furthermore, the compound was appeared to induce autophagy.

我发现了一种小的有机分子，它能在哺乳动物细胞中诱导出巨大的空泡。本研究的目的是以该化合物为工具阐明液泡化的分子机制。为了研究空泡的起源，我进行了免疫荧光显微镜研究，发现AAE 1和M6PR，早期和晚期内体标记蛋白，分别定位，与正常状态下的不同。我发现核内体由于相互融合而增大。此外，该化合物似乎诱导自噬。

项目成果

Small-molecule fluorescent probes for specific RNA targets

• DOI: 10.1039/c1cc10393h
• 发表时间: 2011-01-01
• 期刊: CHEMICAL COMMUNICATIONS
• 影响因子: 4.9
• 作者: Murata, Asako;Sato, Shin-ichi;Uesugi, Motonari
• 通讯作者: Uesugi, Motonari

Deactivation of STAT6 through Serine 707 Phosphorylation by JNK

• DOI: 10.1074/jbc.m110.168435
• 发表时间: 2011-02-04
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Shirakawa, Takashi;Kawazoe, Yoshinori;Uesugi, Motonari
• 通讯作者: Uesugi, Motonari

A novel screen using the Reck tumor suppressor gene promoter detects both conventional and metastasis-suppressing anticancer drugs

使用 Reck 肿瘤抑制基因启动子的新型筛选可检测常规抗癌药物和抑制转移的抗癌药物

• 发表时间: 2010
• 期刊: Oncotarget
• 作者: Murai R;Yoshida Y;Muraguchi T;Nishimoto E;Morioka Y;Kitayama H;Kondoh S;Kawazoe Y;Hiraoka M;Uesugi M;Noda M
• 通讯作者: Noda M

Deactivation of STAT6 through serine 707 phosphorilation by JNK.

JNK 通过丝氨酸 707 磷酸化使 STAT6 失活。

• 发表时间: 2011
• 期刊: J.Biol.Chem. 286
• 作者: Shirakawa;T.;Kawazoe;Y.;Tsujikawa;T.;Jung;D.;Sato;S.;Uesugi;M.
• 通讯作者: M.

A Small Molecule That Blocks Fat Synthesis By Inhibiting the Activation of SREBP

• DOI: 10.1016/j.chembiol.2009.07.007
• 发表时间: 2009-08-28
• 期刊: CHEMISTRY & BIOLOGY
• 作者: Kamisuki, Shinji;Mao, Qian;Uesugi, Motonari
• 通讯作者: Uesugi, Motonari