Analysis of vacuole formation by small organic molecules

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I discovered a small organic molecule that induces a huge vacuoles in mammalian cells. The aim of this study is elucidating a molecular mechanism of vacuolation by means of this compound as a tool. To examine the vacuole origin, I conducted a immuno-fluorescense microscopic study and found that localizations of AAE1 and M6PR, early and late endosome marker protein, respectively, was different from those under normal state. And I found that endosomes are enlarged by fusion each other. Furthermore, the compound was appeared to induce autophagy.