光応答性誘導体化試薬の開発と高精度分離分析への適用

光响应衍生化试剂的研制及其在高精度分离分析中的应用

• 批准号: 20659006
• 负责人: 大庭 義史
• 金额: $ 2.05万
• 依托单位: Nagasaki International University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20659006/
• 关键词: 分析化学; クロマトグラフィー; フォトクロミック化合物; 電気泳動; 誘導体化; 分析科学; 薬学; 光物性; 有機化学

高速液体クロマトグラフィー(HPLC)は、医薬品、生体成分、環境成分等の分離における有用な手段であるが、極微量成分の高感度検出を行う際、測定対象物質を夾雑成分から更なる高精度に分離することが求められる。本研究では、光照射により化学的・物理的性質が変化する化学物質、フォトクロミック化合物を母格として持つ誘導体化試薬を用いる新規分離手法の開発を試みた。この方法では、フォトクロミック化合物で誘導体化された測定対象物の溶出時間を、HPLC移動相の組成を変化させることなく照射する光(可視光と紫外光)を切り替身えることにより制御することで、従来にない測定対象物質の夾雑物質がちお高精度な分離が強く期待できる。前年度に引き続き、光照射から変化するまでにかかる時間、光照射前後における化合物の安定性、溶解性などの点を考慮し、誘導体化指示薬の母核として有用なフォトクロニック化合物のスクリーングを行った。スクリーニングの結果、スピロピラン類が有用と思われこれら化合物について、HPLCを用いて可視光および紫外光照射による分離挙動の変化について調査を行った。当初は透過性チューブに充てん剤を封入したものをカラムとして利用する考えであったが、短カラム、透過性チューブ(光照射部位)、分離カラムの組合せでの分離を行っている。一方、母格への測定対象花谷物に対する反応部位の導入については、精製等の問題があり、現在継続して合成を試みている。

High speed liquid chromatography (HPLC) is a useful method for separating pharmaceutical, biological and environmental components with high sensitivity and determination of fine dust components. In this study, the development of new separation methods for chemical and physical properties of chemical substances and compounds was studied. This method is expected to provide high accuracy in the determination of the dissolution time of the target substance, the composition of the HPLC mobile phase, the detection of the target substance by irradiation with light (visible light and ultraviolet light), the detection of the target substance and the separation of the target substance. The stability and solubility of the compound before and after light irradiation should be considered. The parent core of the induction indicator should be used for the preparation of the compound. The results of this study were as follows: (1) The results of the study were as follows: (2) The results of the study were as follows: (3) The results of the study were as follows: (4) The results of the study were as follows: (5) The results of the study were as follows: (6) The results of the study were as follows: (1) The results of the study were as follows: (1) The results of the study were as follows: (2) The results of the study were as follows: (3) The results of the study were as follows: (4) The results of the study were as follows: (5) The results of the study were as follows: (6) The results of the study was as follows: (7) The results of the study was as follows: (8) The results of the study was as follows: (9) The results of the study was as follows: (8) The results of the study was as follows: (9) The results of the study was as follows: (8) The results of the study was as follows: (9) The results of the study was as follows: ( In the first place, the transparent film is fully enclosed, and the separation of the film is carried out by the combination of the film, the short film, the transparent film (light irradiated part), and the separation film. The determination of a square, mother cell, etc. for the introduction of reverse parts of the grain, such as the problem of refining, now try to synthesize

项目成果

A validated HPLC-fluorescence method with a semi-micro column for routine determination of homocysteine, cysteine and cysteamine, and the relation between the thiol derivatives in normal human plasma.

• DOI: 10.1002/bmc.1205
• 发表时间: 2009-09
• 期刊: Biomedical chromatography : BMC
• 作者: S. Ichinose;Mayumi Nakamura;Mayu Maeda;Rie Ikeda;M. Wada;M. Nakazato;Y. Ohba;N. Takamura;T. Maeda;K. Aoyagi;K. Nakashima