Molecular basis of self/non-self recognition in self-incompatibility on cruciferous plants

在本项目中，我首先尝试通过自交不亲和突变体与QTL分析相结合的方法，分离下游自交不亲和（SI）信号相关的突变基因。我在几个染色体上发现了调控SI下游的QTL。其次，通过对拟南芥品系的调查，我发现了SP11基因在雄性S决定因素上的反转。将修复的反转SP11引入拟南芥中，成功取到SI拟南芥系，并将数据发表在国际期刊《Nature》上。作为其他课题，我利用激光显微解剖和下一代测序仪建立了在乳头细胞上表达的基因目录。为了表征花粉-柱头相互作用的动态，我建立了延时成像系统，用显微镜观察。

In this project, at first, I tried to isolate mutated genes related to self-incompatibility (SI) signaling downstream by using combination between self-compatible mutants and QTL analysis. I identified QTL regulating SI downstream on several chromosomes. Secondly, from surveying Arabidopsis lines, I identified inversion on SP11 gene functioning male S determinant. When repaired SP11 on inversion was introduced into Arabidopsis, I succeeded to take SI Arabidopsis line, and published the data on International Journal “Nature". As other topics, I set up gene catalog expressing on papilla cell by using laser microdissection and next generation sequencer. For characterizing pollen-stigma interaction dynamics, I established time-lapse imaging system for observation by microscope.