Molecular basis of self/non-self recognition in self-incompatibility on cruciferous plants

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In this project, at first, I tried to isolate mutated genes related to self-incompatibility (SI) signaling downstream by using combination between self-compatible mutants and QTL analysis. I identified QTL regulating SI downstream on several chromosomes. Secondly, from surveying Arabidopsis lines, I identified inversion on SP11 gene functioning male S determinant. When repaired SP11 on inversion was introduced into Arabidopsis, I succeeded to take SI Arabidopsis line, and published the data on International Journal “Nature". As other topics, I set up gene catalog expressing on papilla cell by using laser microdissection and next generation sequencer. For characterizing pollen-stigma interaction dynamics, I established time-lapse imaging system for observation by microscope.