Molecular genetic dissection of anovel unilateral incompatibility within species, which observed in self-incompatible Brassica species

自交不亲和（SI）系统通过排斥来自具有相同单倍型的植物的花粉来防止自交受精并促进异交。芸苔属SI系统在孢子体上由单个位点的多个等位基因控制，称为S位点。在芸苔属植物花粉-柱头互作研究中，虽然种间识别系统应该存在，但种间识别分子尚不清楚。在芸苔属植物SI的遗传分析过程中，我们在芜菁中发现了一种新的单侧不亲和性（UI）。当一个栽培品种（Osome）被用作雌性伴侣，而土耳其品系被用作花粉伴侣时，特别观察到这种UI。遗传分析表明，该US位点与S位点无关。本研究的目的是在B中克隆该UI基因。从更深层次的遗传分析来看，花粉UI（PUI）受单隐性基因控制，柱头UI（SUI）受单显性基因控制。为了分离PUI基因，利用F2分离群体进行AFLP分析。分离到6个连锁分子标记，分布在遗传图谱的18 cM范围内。通过遗传图谱和UI表型的比较，我们进行了QTL分析。PUI位于E-AAC/M-CCT和E-ACG/M-GAC标记之间（5cM）。利用这些标记，我们将分离PUI基因，不久的将来。

Self-incompatibility (SI) systems prevent self-fertilization and promote out-crossing by rejecting pollen from plants with the same Shaplotype. The Brassica SI system is controlled sporophytically by multiple alleles at the single locus, termed S locus. On the pollen-stigma interaction of Brassica species, although the recognition system between species should be existed, the recognition molecules between species are unknown. In the course of genetic analysis of SI in Brassica species, we observed a novel unilateral incompatibility (UI) in Brassica rapa L. This UI was specifically observed when one cultivar (Osome) was used a female partner, and Turkish lines were used as a pollen partner. From genetic analysis, this US locus was independent to S locus. This research purpose is molecular cloning of this UI gene in B. rapa.From more deep genetic analysis, pollen UI (PUI) phenotype was controlled by a single recessive gene, and stigma UI (SUI) phenotype was controlled by a single dominant gene. For isolation of PUI gene, AFLP analysis was performed with F2 segregation population. As a result, six linking molecular markers were isolated, and were scattered within 18cM on the genetic map. From the comparison of genetic map and UI phenotype, we performed QTL analysis. In the analysis, PUI was located between E-AAC/M-CCT and E-ACG/M-GAC markers (5cM). By using these markers, we will isolate the PUI gene, near future.