Molecular genetic dissection of anovel unilateral incompatibility within species, which observed in self-incompatible Brassica species

在自交不亲和的芸苔属物种中观察到的物种内新的单方面不相容性的分子遗传学解析

• 批准号: 17380001
• 负责人: WATANABE Masao
• 金额: $ 10.81万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17380001/
• 关键词: Brassica species; Self-incompatibility; Unilateral incompatibility; Pollen-stigma interaction; Genetic map; Molecular marker; pollen factor of UI; stigma factor of UI

Self-incompatibility (SI) systems prevent self-fertilization and promote out-crossing by rejecting pollen from plants with the same Shaplotype. The Brassica SI system is controlled sporophytically by multiple alleles at the single locus, termed S locus. On the pollen-stigma interaction of Brassica species, although the recognition system between species should be existed, the recognition molecules between species are unknown. In the course of genetic analysis of SI in Brassica species, we observed a novel unilateral incompatibility (UI) in Brassica rapa L. This UI was specifically observed when one cultivar (Osome) was used a female partner, and Turkish lines were used as a pollen partner. From genetic analysis, this US locus was independent to S locus. This research purpose is molecular cloning of this UI gene in B. rapa.From more deep genetic analysis, pollen UI (PUI) phenotype was controlled by a single recessive gene, and stigma UI (SUI) phenotype was controlled by a single dominant gene. For isolation of PUI gene, AFLP analysis was performed with F2 segregation population. As a result, six linking molecular markers were isolated, and were scattered within 18cM on the genetic map. From the comparison of genetic map and UI phenotype, we performed QTL analysis. In the analysis, PUI was located between E-AAC/M-CCT and E-ACG/M-GAC markers (5cM). By using these markers, we will isolate the PUI gene, near future.

自交不亲和（SI）系统通过排斥来自具有相同单倍型的植物的花粉来防止自交受精并促进异交。芸苔属SI系统在孢子体上由单个位点的多个等位基因控制，称为S位点。在芸苔属植物花粉-柱头互作研究中，虽然种间识别系统应该存在，但种间识别分子尚不清楚。在芸苔属植物SI的遗传分析过程中，我们在芜菁中发现了一种新的单侧不亲和性（UI）。当一个栽培品种（Osome）被用作雌性伴侣，而土耳其品系被用作花粉伴侣时，特别观察到这种UI。遗传分析表明，该US位点与S位点无关。本研究的目的是在B中克隆该UI基因。从更深层次的遗传分析来看，花粉UI（PUI）受单隐性基因控制，柱头UI（SUI）受单显性基因控制。为了分离PUI基因，利用F2分离群体进行AFLP分析。分离到6个连锁分子标记，分布在遗传图谱的18 cM范围内。通过遗传图谱和UI表型的比较，我们进行了QTL分析。PUI位于E-AAC/M-CCT和E-ACG/M-GAC标记之间（5cM）。利用这些标记，我们将分离PUI基因，不久的将来。

项目成果

Anther-specific genes, which expressed through microsporogenesis, are temporally and spatially regulated in model legume, Lotus japonicus

• DOI: 10.1266/ggs.81.57
• 发表时间: 2006-02-01
• 期刊: GENES & GENETIC SYSTEMS
• 影响因子: 1.1
• 作者: Masuko, Hiromi;Endo, Makoto;Watanabe, Masao
• 通讯作者: Watanabe, Masao

Comparative analysis of the S-intergenic region in the class-II S haplotypes of self-incompatible Brassica rapa (syn. campestris).

自交不亲和白菜（syn.campestris）II类S单倍型中S-基因间区域的比较分析。

• 发表时间: 2006
• 期刊: Genes Genet.Syst. 81(in press)
• 作者: Kakizaki;T.
• 通讯作者: T.

Molecular characterization of two pollen-specific genes encoding putative RNA-binding Proteins, AtRBP45s, in Arabidopsis thalianal

拟南芥中两个编码假定 RNA 结合蛋白 AtRBP45s 的花粉特异性基因的分子特征

• 发表时间: 2006
• 期刊: Genes Genet. Syst. 81
• 作者: Park ;et. al.
• 通讯作者: et. al.

Identification and characterization of new small RNAs associated with male organ differentiation in rice.

与水稻雄性器官分化相关的新小RNA的鉴定和表征。

• 发表时间: 2008
• 作者: Fujioka ;et. al.
• 通讯作者: et. al.

花の不思議な世界-リンゴはどうやってあんな風に赤く食べられるようになるの??-

神秘的花的世界——苹果是怎么变得这么红并且可以吃的？

• 发表时间: 2007
• 作者: 川岸万紀子;ら;渡辺正夫
• 通讯作者: 渡辺正夫