Molecular basis of mechanisms of dominance relationships among S haplotypes in self-incompatible Brassicaceae.

自交不亲和十字花科S单倍型之间显性关系机制的分子基础。

• 批准号: 14360002
• 负责人: WATANABE Masao
• 金额: $ 9.28万
• 依托单位: Iwate University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14360002/
• 关键词: Cruciferous plants; Self-incompatibility; self; non-self recognition; S multiple alleles; Dominance relationships; SP11 gene; Methylation; Regulation of gene expression

Self-incompatibility (SI) systems prevent self-fertilization and promote out-crossing by rejecting pollen from plants with the same S haplotype. The Brassica SI system is controlled sporophytically by multiple alleles at the single locus, termed S locus. Because of sporophytic control of Brassica SI, dominance relationships among S haplotypes are observed in both stigma and pollen. We performed test-crosses between S heterozygotes and their respective parental S homozygotes for four of these class-II S haplotypes, and observed a linear dominance relationship on the pollen side. In order to determine how this dominance relationship is controlled, RNA gel blot analyzes were performed for six S heterozygotes and their respective parental S homozygotes by using the corresponding SP11 cDNA clone as a probe. In all six S heterozygotes, SP11 derived from a dominant S haplotype was predominantly expressed, and SP11 derived from a recessive S haplotype was repressed. Therefore, the linear dominance relationship of the SI phenotype on the pollen side is regulated by the expression of SP11 gene.We determined the nucleotide sequences of the genomic clone of three class-II S haplotypes that contained corresponding SP11. These contain the part of the 5'-flanking region of the respective SRK. The distance of SP11 and SRK is about 10kbp in each S haplotypes. This result indicates that the relative position of SP11 and SRK is conserved. Therefore, the region between SP11 and SRK was named the "S-intergenic region". Then, we cloned class-II SRK, and compared these with other class-II SRK. The predicted amino acid sequence similarity within class-II SRK is about 90 to 95%, though the hypervariable region of the S domain is diverted. These results indicate not only similarity of S genes but that the physical position of their SP11 and SRK is conserved in the class-II S haplotypes.

自交不亲和(SI)系统通过拒绝来自具有相同S单倍型的植物的花粉来防止自交受精和促进异交。甘蓝型油菜的SI系统是由同一座位上的多个等位基因控制的，称为S基因座。由于孢子体控制，柱头和花粉中均观察到S单倍型间的显性关系。我们用S杂合子及其亲本S纯合子对其中4个S单倍型进行了测交，观察到花粉侧存在线性显性关系。为了确定这种显性关系是如何控制的，以相应的SP11cDNA克隆为探针，对6个S杂合子及其相应的亲本S纯合子进行了RNA凝胶印迹分析。在所有6个S杂合子中，来自显性S单倍型的SP11显著表达，而来自隐性S单倍型的SP11被抑制。因此，花粉侧SI表型的线性显性关系受SP11基因表达的调节。我们测定了三个含有相应SP11的S二类单倍型基因组克隆的核苷酸序列。它们包含各自SRK的5‘-侧翼区的一部分。在每个S单倍型中，SP11和SRK的距离约为10kbp。这一结果表明，SP11和SRK的相对位置是保守的。因此，SP11和SRK之间的区域被命名为“S-基因间隔区”。然后，我们克隆了第二类SRK，并将其与其他第二类SRK进行了比较。虽然S结构域的高变区被转移，但预测的氨基酸序列在II类SRK中的相似性约为90%至95%。这些结果不仅表明S基因的相似性，而且其SP11和SRK在S二级单倍型中的物理位置是保守的。

项目成果

Dominant/recessive relationship in pollen expression of self-incompatibility is determined by the transcriptional level of the pollen determinant gene in the S-heterozygotes of Brassica.

自交不亲和性花粉表达的显性/隐性关系由芸苔属S杂合子中花粉决定基因的转录水平决定。

• 发表时间: 2002
• 期刊: Plant Cell 14
• 作者: Shiba;H.
• 通讯作者: H.

渡辺正夫: "アブラナ科植物の自家不和合性の認識反応を制御する遺伝子の分子生物学的解剖"The Annals of Intelligent Cosmos Academic Foundation. 8. 13-16 (2003)

Masao Watanabe：“控制十字花科自交不亲和性识别反应的基因的分子生物学解剖”智能宇宙学术基金会年鉴 8. 13-16 (2003)。

Isolation of a cDNA for a nucleoside diphosphate kinase capable of phosphorylating the kinase domain of the self-incompatibility fator SRK of Brassica campestris.

能够磷酸化甘蓝自交不亲和因子SRK的激酶结构域的核苷二磷酸激酶的cDNA的分离。

• 发表时间: 2002
• 期刊: J.Exp.Bot. 53
• 作者: Matsushita;Y.
• 通讯作者: Y.

Identification of S-alleies using PCR-CAPS of the S-locus receptor kinase (SRK) gene in breeding lines of Brassica oleracea.

使用甘蓝育种系中 S 位点受体激酶 (SRK) 基因的 PCR-CAPS 鉴定 S 通道。

• 发表时间: 2002
• 期刊: Plant Breed. 121
• 作者: Park;J.-I.
• 通讯作者: J.-I.

Tandem duplication of SP11 gene at the S locus of S15 haplotype in Brassica oleracea.

甘蓝 S15 单倍型 S 基因座上 SP11 基因的串联重复。

• 发表时间: 2004
• 期刊: Genes Genet.Syst. 79
• 作者: Shiba;H.
• 通讯作者: H.