Integrated research for the mechanism of sleep/wakefulness regulation
Integrated research for the mechanism of sleep/wakefulness regulation
批准号:
20680021
负责人:
YAMANAKA Akihiro
金额:
$13.23万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Young Scientists (A)
财政年份:
2008
资助国家:
日本
项目状态:
已结题
起止时间:
2008 至 2010
中文摘要
睡眠/觉醒是一种仅在整个身体中观察到的生理现象。因此,有必要使用整个动物进行研究。光遗传学是最近开发的一项技术,通过表达光激活蛋白(例如视紫红质通道蛋白 2 (ChR2) 或盐视紫红质 (Halo))来控制神经元的活动。这项技术使我们能够在体内和体外利用光控制产生食欲素的神经元(食欲素神经元)的活动。首先,我们使用切片膜片钳确认了食欲素神经元中CHR2和Halo的功能,并确定了光照条件。接下来,我们使用有意识的小鼠进行体内实验。将光纤双侧植入下丘脑。通过光纤将橙色光照射到下丘脑,可以抑制食欲素神经元的活动。同时,中缝核中血清素能神经元的活动被细胞外记录下来。食欲素神经元的急性抑制诱导慢波睡眠,并且血清素能神经元的活性随着慢波睡眠的开始而降低。这一结果表明,抑制食欲素神经元可诱导小鼠慢波睡眠。
英文摘要
Sleep/wakefulness is a physiological phenomenon which observed only in the whole body. Thus, it is essential to study using whole animal. Optogenetics is a recently developed technique to control the activity of neurons by expressing light activated proteins, such as channelrhodopsin2(ChR2) or halorhodopsin(Halo). This technique allows us to control the activity of orexin-producing neurons(orexin neurons) using light in vivo and in vitro. First, we confirmed the function of CHR2 and Halo in the orexin neurons using slice patch clamp and decided a condition of light illumination. Next, we proceeded in vivo experiment using conscious mouse. Optical fibers were bilaterally implanted into the hypothalamus. The activity of orexin neurons was inhibited by illuminating orange light into the hypothalamus through the optical fibers. At the same time, the activity of serotonergic neurons in the raphe nucleus is recorded by the extracellular recordings. Acute inhibition of orexin neurons induced slow wave sleep and the activity of serotonergic neurons was decreased in accordance with initiation of slow wave sleep. This result showed that inhibition of orexin neurons induced slow wave sleep in mice.
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バソプレシンはVla受容体を介してオレキシン神経を活性化する
加压素通过 Vla 受体激活食欲素神经元
DOI:
--
发表时间:
2008
期刊:
影响因子:
--
作者:
[常松友美, 山中章弘, 富永真琴]
通讯作者:
富永真琴
DOI:
10.1093/sleep/34.1.31
发表时间:
2011-01-01
期刊:
SLEEP
影响因子:
5.6
作者:
[Hirashima, Noriko, Tsunematsu, Tomomi, Yamanaka, Akihiro]
通讯作者:
Yamanaka, Akihiro
オレキシン神経による睡眠覚醒調節
食欲素神经的睡眠-觉醒调节
DOI:
--
发表时间:
2010
期刊:
影响因子:
--
作者:
[山中章弘, 常松友美]
通讯作者:
常松友美
An artificial control of sleep/wakefulness state using optogenetics in mice
利用小鼠光遗传学人工控制睡眠/觉醒状态
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[Yamanaka A, Tsunematsu T]
通讯作者:
Tsunematsu T
睡眠覚醒調節におけるオレキシン神経とセロトニン神経の役割について
食欲素和血清素能神经元在睡眠/觉醒调节中的作用
DOI:
--
发表时间:
2010
期刊:
影响因子:
--
作者:
[Yamanaka A, Tsunematsu T, Tabuchi S, Tominaga M]
通讯作者:
Tominaga M
共 22 条
Development of method for neural activity manipulation using melanopsin
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批准号:23659128
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项目类别:Grant-in-Aid for Challenging Exploratory Research
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资助金额:$2.41万
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财政年份:2011
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负责人:YAMANAKA Akihiro
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依托单位:
Study for neural mechanism of wakefulness and dependence using optogenetics
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批准号:23300142
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$12.9万
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财政年份:2011
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负责人:YAMANAKA Akihiro
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依托单位:
海外基金