Chemical synthesis of unique AG-gangliosides and evaluations of nerve cell stimulation activities

独特的 AG-神经节苷脂的化学合成和神经细胞刺激活性的评估

• 批准号: 20710171
• 负责人: HANASHIMA Shinya
• 金额: $ 2.16万
• 依托单位: The Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20710171/
• 关键词: ガングリオシド; 合成; シアル酸; NMR; Siglec-2; 糖脂質; 糖鎖; グリコシル化; 有機合成; 神経突起伸長

Gangliosides isolated from starfish have unique glycan sequences and nerve cell stimulating activities. We focused on their unique glycan sequences and synthesized the glycan parts by developing novel sialylation strategies. Further, we analyzed the binding between a starfish glycan and a protein Siglec, expressing on mammalian cell surfaces and modulating their functions. We elucidated the binding epitope as the trisaccharide unit including the sialic acid residue. Our finding revealed the potential that inner sialic acid could be also recognized by Siglec, which would develop useful potential for inhibitors of Siglec.

从海星中分离的神经节苷脂具有独特的聚糖序列和神经细胞刺激活性。我们专注于其独特的聚糖序列，并通过开发新的唾液酸化策略合成聚糖部分。此外，我们分析了海星聚糖和蛋白质Siglec之间的结合，在哺乳动物细胞表面表达并调节其功能。我们将结合表位阐明为包括唾液酸残基的三糖单元。我们的发现揭示了内唾液酸也可以被Siglec识别的潜力，这将为Siglec的抑制剂开发有用的潜力。

项目成果

Synthesis and biological function of the unique starfish ganglioside AG2 pentasaccharide Austria/Japan seminar on Comparative Glycobiology and Development Biology

独特的海星神经节苷脂AG2五糖的合成和生物学功能奥地利/日本比较糖生物学和发育生物学研讨会

• 发表时间: 2009
• 作者: Shinya Hanashima

Synthesis of the starfish ganglioside LLG-3 tetrasaccharide

• DOI: 10.1016/j.carres.2009.02.003
• 发表时间: 2009-04-21
• 期刊: CARBOHYDRATE RESEARCH
• 影响因子: 3.1
• 作者: Hanashima, Shinya;Ishikawa, Daichi;Sato, Ken-ichi
• 通讯作者: Sato, Ken-ichi

Silylene/Oxazolidinone Double-Locked Sialic Acid Building Blocks for Efficient Sialylation Reactions in Dichloromethane

用于二氯甲烷中高效唾液酸化反应的硅烯/恶唑烷酮双锁唾液酸结构单元

• 发表时间: 2009
• 期刊: European Journal of Organic Chemistry
• 影响因子: 2.8
• 作者: 荒川和晴;冨田勝;Shinya Hanashima;荒川和晴;Shinya Hanashima
• 通讯作者: Shinya Hanashima

Synthesis of the unique starfish ganglioside AG2 pentasaccharide using silylene/oxazolidinone double-locked building blocks

使用亚甲硅基/恶唑烷酮双锁结构单元合成独特的海星神经节苷脂 AG2 五糖

• 发表时间: 2009
• 作者: 花島慎弥

Synthesis and biological function of the unique starfish ganglioside AG2 pentasaccharide

独特的海星神经节苷脂AG2五糖的合成及其生物学功能

• 发表时间: 2009
• 作者: A. Farstner;L. C. Bouchez;L. Morency;J. -A. Funel;V. Liepins;F. -H. Pored;R. Gilmour;F. Eleaufi Is;D. Laurich;F. BeaufiIs;M. Tamiya;花島慎弥
• 通讯作者: 花島慎弥