Transportation and Fusion of Donor Cell or Oocyte-Laden MultiphaseEmulsion Droplet On a Chip

这项工作的目标是通过液滴分配技术在芯片上产生单个卵母细胞或供体细胞负载的液滴。本研究的新颖之处在于利用磁性微致动器（MMT）在芯片上实现卵母细胞与供体细胞的融合，从而产生载粒子液滴。主要工作成果如下：(1)阀功能MMT成功切割液滴，制备出粒径可控的液滴，并包裹一个粒径为50 μm的颗粒，并逐一输送。因此，在芯片上的液滴中可以包含各种大小的颗粒或细胞。(2)采用高精度镍金属电镀技术成功制备了杂化型MMT。成功地控制了MMT的局部刚度和磁性能，获得了比常规设置大6倍的位移。改进后的芯片可以具有层流护套流动或压力平衡，从而成功地在芯片上按需生产液滴。

This work is targeting to produce a single oocyte or donor cell laden droplet on a chip by means of droplet dispensing techniques. The novelty of the present work is that we produce particle-laden droplet by using magnetic micro-actuator (MMT) to achieve the fusion of the oocyte and donor cell on a chip. The main achievement of the work is summarized as follows :(1) Valve-functioned MMT was successfully chopped droplet and produced size-controlled droplet together with enclosing a particle whose size of 50 μm and transported them one by one. Therefore a wide range of the size of particles or cells can be enclosed in a droplet on a chip.(2) Hybrid type MMT was successfully fabricated by using high-accuracy Ni metal by electroplating technique. Local rigidity and magnetic property of MMT was successfully controlled and obtained 6 times displacement as large as conventional setting. The revised design chip could have laminar sheath flow or pressure balance which provided on-demand droplet production on a chip successfully.