Elucidation of the natural healing mechanism after knee anterior cruciate ligament tear and it's clinical application

膝关节前交叉韧带撕裂自然愈合机制的阐明及其临床应用

• 批准号: 21500484
• 负责人: TAKAYANAGI Kiyomi
• 金额: $ 2.91万
• 依托单位: Saitama Prefectural University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21500484/
• 关键词: 前十字靭帯; 保存療法; 動物実験モデル; 膝前十字靭帯; 自然治癒; 関節制動; リアルタイムPCR法; タンパク質分解酵素; mRNA; MMP-13; TIMP-1; 前十字靱帯; 靱帯治癒; 靱帯強度; 大腿骨後方-脛骨前モデル

The natural healing of the ruptured anterior cruciate ligament(ACL) using a new animal model was observed by mechanical, histological and immunohistochemistrical methods. The ACL was cut completely by the mini-scissors and then the knee joint was controlled normally with artificial ligament. The ruptured ACL was healed in all rats. Eight weeks after ACL cutting, the Type III collagen was increased in the healing ligament. After 8 weeks and 40 weeks after the ACL cut, the healing ligament was half strength compared to the normal ligament.The expression of MMP-1, MMP-13 and TIMP-1 in medial and lateral meniscus and rupture ACL was increased after ACL cut. Expression of MMP-13 in the joint control group has been suppressed compared to the no joint control group. It is suggested that abnormal joint movement under the gravity is increased to express of proteolytic enzymes of both meniscus and rupture ACL in the knee joint.

应用力学、组织学和电化学方法，观察了一种新的前交叉韧带（ACL）断裂动物模型的自然愈合情况。用微型剪刀完全切断ACL，然后用人工韧带正常控制膝关节。所有大鼠的ACL断裂均愈合。ACL切割后8周，愈合韧带中的III型胶原蛋白增加。ACL切断后8周和40周，愈合韧带强度为正常韧带的一半，内、外侧半月板和断裂ACL中MMP-1、MMP-13和TIMP-1的表达均增加。与无关节对照组相比，关节对照组中MMP-13的表达受到抑制。提示重力作用下关节运动异常增加了膝关节半月板和前交叉韧带断裂处蛋白水解酶的表达。

项目成果

Controlling anomalous motion leads anterior cruciate ligament

控制异常运动导致前十字韧带

• 发表时间: 2011
• 作者: Kokubun T.;Kanemura N;Takayanagi K.
• 通讯作者: Takayanagi K.

Biomechanical and histological effects of weight bearing on anterior cruciate ligament following unloading

卸载后负重对前十字韧带的生物力学和组织学影响

• 发表时间: 2011
• 作者: Kanemura N;Takayanagi K.;et al
• 通讯作者: et al

The manufacture of the anterior cruciate ligament reproduct model

前交叉韧带复制模型的制作

• 发表时间: 2010
• 作者: Takayanagi K;Kokubun T;Kanemura N;et al
• 通讯作者: et al

The manufacture of the anteror cruciate ligament reproduction model

前交叉韧带复制模型的制作

• 发表时间: 2010
• 作者: Takayanagi K;Kokubun T;Kanemura N;et al;高柳清美
• 通讯作者: 高柳清美