Measurement of Oxidative Stress on a single cell level by the beam deflection method and capillary electrophoresis

这项研究旨在开发一种测量单细胞氧化应激的新方法。首先，通过探针束的偏转法，监测和比较了单个细胞在氧化应激前后的行为，例如通过添加过氧化氢溶液。然后，用自制的毛细管电泳法-激光诱导荧光检测系统对细胞内的活性氧进行分析。采用鲁米诺-化学发光法对细胞内的活性氧进行了定量分析。以培养的HeG2细胞作为模型细胞样本。

This research aimed at developing a novel method for measurement of oxidative stress in a single cell. Firstly, behavior of a single cell before and after an oxidative stress such as by addition of a hydrogen peroxide solution was monitored and compared by the deflection method of a probe beam. Then, analysis of active oxygen species in cells was carried out by a home-made capillary electrophoresis-laser induced fluorescence detection system. The luminol-chemliuminescence method was further used for quantitative analysis of active oxygen species in cells. HeG2 cell was cultured and used as a model cell sample.