Structural and molecular genetical analyses of factors related to bacterial chromosome condensation

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I have identified functional regions of regulator subunits(ScpA and ScpB) of the bacterial condensin, and investigated their biochemical properties. In addition, using various mutants of the subunits, I examined the ATPase activity of the SMC subunit. As a result, some peptide regions of ScpA, through binding of the C-terminal domain of ScpB, control the ATPase activity of the SMC subunit. This result suggests that internal conformational changes of the regulatory subcomplex organize dimerization of the SMC subunit in the bacterial condensin.