Studies on the mechanism of viroplasm formation during plant negative-strand RNA virus infection

兰花斑点病毒（Orchid fleck virus， OFV）是一种独特的双节段负义RNA病毒，在被感染的植物细胞中诱导核内电子发光病毒质（包涵体）。我们研究了OFV病毒质在体内产生的分子机制。农杆菌浸润法的瞬时表达检测显示，在没有病毒感染的情况下，核衣壳蛋白N和推定的磷蛋白P的共表达足以在烟叶细胞中形成核内电子发光的病毒质样结构。

Orchid fleck virus(OFV), a unique two-segmented negative-sense RNA virus, induces an intranuclear electron-lucent viroplasm(inclusion) in infected plant cells. We studied the molecular mechanism by which OFV viroplasms are produced in vivo. Transiently expression examinations by Agrobacterium-infiltration reveled that coexpression of nucleocapsid protein N and the putative phosphoprotein P, in the absence of virus infection, was sufficient for the formation of an intranuclear electron-lucent viroplasm-like structure in Nicotiana benthamiana cells.