Analysis of substrate-recognition mode by novel ubiquitin ligase related to endoplasmic reticulum-associated degradation pathway.

与内质网相关降解途径相关的新型泛素连接酶的底物识别模式分析。

• 批准号: 21580121
• 负责人: YOSHIDA Yukiko
• 金额: $ 3.16万
• 依托单位: 財団法人東京都医学総合研究所 (2011)Tokyo Metropolitan Organization for Medical Research (2009-2010)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21580121/
• 关键词: F-box蛋白質; ユビキチンリガーゼ; 小胞体関連分解; N型糖鎖; 基質認識; 糖鎖; 基質認識機構

We have reported previously that Fbs1 and Fbs2 are glycoprotein-specific F-box proteins, which are identified as the endoplasmic reticulum-associated degradation(ERAD)-linked ubiquitin ligase component. Here we report that Fbxo44b, which does not have sugar-binding activity, is involved in ubiquitylation of glycoproteins under the ERAD pathway. In addition, we analyzed the third F-box protein recognizing N-glycoproteins, Fbs3.Fbs3 can interact with glycoproteins modified with not only high-mannose but also complex-type glycans, suggesting that participates in unknown function of glycoproteins endocytosed from the cell surface but not through the ERAD pathway.

我们已经报道了Fbs1和Fbs2是糖蛋白特异性的F-box蛋白，它们被鉴定为内质网相关降解(ERAD)连接的泛素连接酶组分。在这里，我们报道了Fbxo44b，它不具有糖结合活性，参与ERAD途径中糖蛋白的泛素化。此外，我们分析了识别N-糖蛋白的第三个F-box蛋白Fbs3。Fbs3不仅可以与高甘露糖修饰的糖蛋白相互作用，还可以与复合型糖链修饰的糖蛋白相互作用，这表明Fbs3参与了从细胞表面内吞的糖蛋白的未知功能，但不是通过ERAD途径。

项目成果

In vivo function of glycoprotein-specific F-box proteins

糖蛋白特异性 F-box 蛋白的体内功能

• 发表时间: 2010
• 作者: Fukunaga K;Fujikawa Y;Esaka M;吉田雪子
• 通讯作者: 吉田雪子

Crystallization and preliminary X-ray characterization of the Skp1-Fbg3 complex

Skp1-Fbg3 复合物的结晶和初步 X 射线表征

• DOI: 10.1107/s1744309109050581
• 发表时间: 2010
• 期刊: Acta Crystallogr. Sect. F. Struct Biol. Cryst. Commun
• 作者: Kumanomidou;T.;et al.(員数9;7番目)
• 通讯作者: 7番目)

SCFFbx2-E3-ligase-mediated degradation of BACE1 attenuates Alzheimer's disease amyloidosis and improves synaptic function.

• DOI: 10.1111/j.1474-9726.2010.00632.x
• 发表时间: 2010-12
• 期刊: Aging cell
• 影响因子: 7.8
• 作者: Gong B;Chen F;Pan Y;Arrieta-Cruz I;Yoshida Y;Haroutunian V;Pasinetti GM
• 通讯作者: Pasinetti GM

Characterization of Fbs3 that is the third N-glycoprotein specific F-box protein

第三个 N-糖蛋白特异性 F-box 蛋白 Fbs3 的表征

• 发表时间: 2011
• 作者: Eltelib HA;Badejo AA;Fujikawa Y;Esaka M.;吉田雪子
• 通讯作者: 吉田雪子