Development of Neutralizing Antibodies against Staphylococcal Exfoliative Toxins

抗葡萄球菌剥脱性毒素中和抗体的研制

• 批准号: 21580387
• 负责人: NISHIFUJI Koji
• 金额: $ 3.08万
• 依托单位: Tokyo University of Agriculture and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21580387/
• 关键词: 感染症; ブドウ球菌; 表皮剥脱毒素; デスモグレイン; 皮膚; 膿痂疹; ブドウ球菌性熱傷様皮膚症候群; ブタ滲出性表皮炎; 細胞接着; 滲出性表皮炎

In this project, we revealed that amino acid motifs critical for substrate specificity of Staphylococcus aureus exfoliative toxin B(ETB) may be located between amino acid residues of 65-232. This project also revealed that two S. pseudintermedius ETs selectively digest canine desmoglein 1, a cell-cell adhesion molecule in canine epidermis.

在本项目中，我们发现金黄色葡萄球菌剥脱毒素B（ET B）的底物特异性的关键氨基酸基序可能位于65-232氨基酸残基之间。该项目还揭示了两个S。pseudintermedius选择性消化犬桥粒芯糖蛋白1，一种犬表皮中的细胞-细胞粘附分子。

项目成果

Alteration of stratum corneum ceramide profiles in spontaneous canine model of atopic dermatitis

• DOI: 10.1111/j.1600-0625.2011.01306.x
• 发表时间: 2011-09-01
• 期刊: EXPERIMENTAL DERMATOLOGY
• 影响因子: 3.6
• 作者: Yoon, Ji-Seon;Nishifuji, Koji;Iwasaki, Toshiroh
• 通讯作者: Iwasaki, Toshiroh

Development of an enzyme-linked immunosorbent assay for detection of circulating IgG autoantibodies against canine desmoglein 3 in dogs with pemphigus

开发酶联免疫吸附测定法，用于检测患有天疱疮的狗体内针对犬桥粒芯糖蛋白 3 的循环 IgG 自身抗体

• 发表时间: 2009
• 期刊: Vet Dermatol 20
• 作者: Iyori K;et al.;杉崎修・小林正人・小堤友香梨・田中優・守谷友加里・石井典子・山田理・礒谷真由・伊藤慶太・盆子原誠・小野憲一郎・鷲巣月美;Kobayashi T;Nishifuji K
• 通讯作者: Nishifuji K

Identification of a novel Staphylococcus pseudintermedius exfoliative toxin gene and its prevalence in isolates from canines with pyoderma and healthy dogs

• DOI: 10.1111/j.1574-6968.2010.02113.x
• 发表时间: 2010-11-01
• 期刊: FEMS MICROBIOLOGY LETTERS
• 影响因子: 2.1
• 作者: Iyori, Keita;Hisatsune, Junzo;Nishifuji, Koji
• 通讯作者: Nishifuji, Koji

Antimicrobial Susceptibility and Methicillin Resistance in Staphylococcus pseudintermedius and Staphylococcus schleiferi subsp coagulans Isolated from Dogs with Pyoderma in Japan

• DOI: 10.1292/jvms.10-0172
• 发表时间: 2010-12-01
• 期刊: JOURNAL OF VETERINARY MEDICAL SCIENCE
• 影响因子: 1.2
• 作者: Kawakami, Tetsuji;Shibata, Sanae;Fukata, Tsuneo
• 通讯作者: Fukata, Tsuneo

Removal of amino-terminal extracellular domains of desmoglein 1 by staphylococcal exfoliative toxin is sufficient to initiate epidermal blister formation

通过葡萄球菌剥脱性毒素去除桥粒芯糖蛋白 1 的氨基末端胞外结构域足以引发表皮水疱形成

• 发表时间: 2010
• 期刊: J Dermatol Sci
• 影响因子: 4.6
• 作者: Jiageng Chen;Shoichi Hirose;Hidenori Kuwakado;and Atsuko Miyaji;Nishifuji K
• 通讯作者: Nishifuji K