Detection of low affinity autoantibodies like anti-amyloid antibodies by surface plasmon resonance analysis Detection of low affinity autoantibodies like anti-amyloid antibodies by surface plasmon resonance analysis

本研究的目的是利用SPR分析（Biacore）开发一种检测低亲和力抗体的方法。作为一个模型，抗淀粉样抗体可能出现在类风湿关节炎的主要并发症aa -淀粉样变性中。在研究过程中，我们成功地建立了抗saa单克隆抗体，包括抗小鼠抗体和抗人抗体。特别是，在淀粉样变过程中由SAA降解产生的针对AA羧基末端的抗体，对于进一步研究AA-淀粉样变的基础和临床研究至关重要。使用Biacore进行抗体检测时，将固定有抗aa抗体的传感器芯片与提取的淀粉样蛋白组织、患者血清和抗人免疫球蛋白依次反应。当使用抗saa抗体代替患者血清时，该系统有效。因此，该系统本身能够用于检测其他自身抗体。然而，使用患者血清的试验未能检测到显著的抗体活性。提高灵敏度可能是下一个需要改进的问题。

The purpose of this study was the development of the method using SPR analysis(Biacore), which could detect low affinity antibodies. As a model, anti-amyloid antibodies, which might appear in AA-amyloidosis, the major complication in rheumatoid arthritis, were targeted. In the process of investigation, we succeeded in establishing monoclonal anti-SAA, either anti-mouse or anti-human, antibodies. Especially, an antibody specific to AA carboxyl-terminus generated from SAA degradation during amyloidogenic process must be useful for the further investigation in fundamental and clinical aspects in AA-amyloidosis. For antibody detection using Biacore, a sensor chip immobilized with anti-AA antibodies was reacted in order with extracted amyloid tissues, patient serum, and anti-human immunoglobulin. The system worked when anti-SAA antibodies were used instead of patient serum. Thus, the system itself is able to be applied for detection of other autoantibodies. However, the trails using patient sera failed to detect the significant antibody activity. To amplify the sensitivity may be the next problem to improve.