Immune targeting of apolipoprotein E in amyloid deposits ; a fundamental study using murine model
Immune targeting of apolipoprotein E in amyloid deposits ; a fundamental study using murine model
批准号:
15590496
负责人:
YAMADA Toshiyuki
金额:
$2.05万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2003
资助国家:
日本
项目状态:
已结题
起止时间:
2003 至 2005
中文摘要
淀粉样变通常在活检标本上进行组织学诊断。然而,这种方法不仅具有侵入性,而且无法评估淀粉样蛋白沉积在全身的数量和定位。已知载脂蛋白(apoE)存在于沉积物中。为了开发新的诊断工具,本研究重点以apoE为靶点。利用人apoE表达小鼠和识别人apoE片段的单克隆抗体。标记抗体在体内与沉积物有效结合,免疫组织化学证实其在沉积物中的定位。然而,高本底放射性不能跟随成像研究。在淀粉样变小鼠中,抗体的血浆半衰期没有缩短。注射抗体并不能阻止小鼠淀粉样变性的发展,也不能增强对已经存在的淀粉样沉积的吸收。这些结果表明,该策略本身是合理的,但可以改进抗体,使其与淀粉样蛋白沉积物具有更强的亲和力。
英文摘要
Amyloidosis is usually diagnosed histologically on biopsy specimens. However, this approach is not only invasive but also is unable to evaluate the amount and localization of amyloid deposits in the whole body. Apolipoprotein (apoE) is known to be present in the deposits. Aiming to develop the new diagnostic tool, in this study, apoE was focused as a target. Human apoE expressing mouse and a monoclonal antibody recognizing human apoE fragment were utilized.Labeled antibody efficiently bound to the deposits in vivo and its localization in the deposits was confirmed immunohistochemically. However, high background of administered radioactivity could not follow the imaging study. The plasma half life of the antibody was not shortened in the amyloidotic mouse. Administration of antibody did not prevent the mouse from amyloidosis development or enhance the absorption of already present amyloid deposits.These results indicate that the strategy itself is reasonable but the antibody may be improved to have the stronger affinity with the amyloid deposits.
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Correlation between serum levels of free light chain and phenotype of plasma-cells in bone marrow in AL amyloidosis.
AL 淀粉样变性患者血清游离轻链水平与骨髓浆细胞表型之间的相关性。
DOI:
--
发表时间:
2005
期刊:
Amyloid : Journal of Protein Folding Disorders 12
影响因子:
--
作者:
[Asai S., et al., 陳 戈林, Gelin CHEN, 陳 戈林, Yuanyuan Xu, Masayuki Matsuda, Yasuhiro shimojima]
通讯作者:
Yasuhiro shimojima
Genetic effects on serum concentrations of serum amyloid A protein (SAA)
遗传因素对血清淀粉样 A 蛋白 (SAA) 血清浓度的影响
DOI:
--
发表时间:
2004
期刊:
Clinical Chemistry 50
影响因子:
--
作者:
[Asai S., et al., 陳 戈林, Gelin CHEN, 陳 戈林, Yuanyuan Xu, Masayuki Matsuda, Yasuhiro shimojima, Masae Kokubun, Tomonosuke Someya, Yasuhiro Shimojima, Matsuda M, Shimojima Y, Kokubun M, Someya T, Toshiyuki Yamada]
通讯作者:
Toshiyuki Yamada
山田俊幸, 奥田恭章: "二次性アミロイドーシスの検出"リウマチ科. 29. 261-265 (2003)
Toshiyuki Yamada、Yasuaki Okuda:“继发性淀粉样变性的检测”风湿病学系 29. 261-265 (2003)。
DOI:
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发表时间:
期刊:
影响因子:
--
作者:
[]
通讯作者:
DOI:
10.1016/j.cccn.2005.04.006
发表时间:
2005-10-01
期刊:
CLINICA CHIMICA ACTA
影响因子:
5
作者:
[Kokubun, M, Imafuku, Y, Yoshida, H]
通讯作者:
Yoshida, H
Yamada T, Wada A: "Slower clearance of human SAA1.5 in mice : Implication for allele specific variation of SAA concentration in human."Amyloid : J Prot Fold Dis. 10. 147-150 (2003)
Yamada T、Wada A:“小鼠体内人类 SAA1.5 的清除速度较慢:对人类 SAA 浓度等位基因特异性变化的影响。”淀粉样蛋白:J Prot Fold Dis。
DOI:
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发表时间:
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作者:
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共 16 条
Development of the Knowledge Cycle between Linguistic Theory and Educational Practice Based on Error Analysis of Japanese Learners of English as a Foreign Language
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Establishment of a new model rat for immunological diseases deleting the Ly49 gene regulating immune reactions
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The Research of the Rejuvenation and Reconstruction of Competitive Advantage in Mature Business
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财政年份:2011
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依托单位:
Detection of low affinity autoantibodies like anti-amyloid antibodies by surface plasmon resonance analysis Detection of low affinity autoantibodies like anti-amyloid antibodies by surface plasmon resonance analysis
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批准号:21590637
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$3.0万
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财政年份:2009
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负责人:YAMADA Toshiyuki
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依托单位:
Functional analysis for serum amyloid A polymorphism
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批准号:18590536
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依托单位:
Isolation and identification of the gene induced by Ets family transcription factor PU.1 in murine erythroleukemia cells
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财政年份:2002
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依托单位:
Analysis of mechanisms of the differentiation inhibition in erythroid cells induced by Ets transcription factor PU. I
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批准号:12671015
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依托单位:
Synthesis, metabolism andfibrillogenesis of serum amyloid A bymonocytes or macrophages
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资助金额:$1.79万
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财政年份:2000
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负责人:YAMADA Toshiyuki
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依托单位:
Isolation of the genes involved in the process of differentiation inhibition and apoptosis induced by PU.1 in murine erythroleukemia cells
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负责人:YAMADA Toshiyuki
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依托单位:
海外基金