Analysis of nonalcoholic fatty liver using androgen receptor transgenic mouse

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We had planned to examine the relationship between androgen receptor and lipid or carbohydrate metabolism using androgen receptor transgenic mouse, but the transgenic mouse could not be produced for examination. Two female transgenic mice produced in the first experiment did not raise their children. A total of 139 mice produced in the next 3 consecutive experiments did not have the transgenic genes. Therefore we transfected androgen receptor expression vector into HepG2 and Huh7, and analyzed the change of m-RNA expression in the 84 genes related to lipid metabolism. Among these genes, the only genes down regulated both in the two cells were Acyl-CoA dehydrogenase and Carnitine palmitoyltransferase 1A, both of which are involved in fatty acid oxidation and the result was contrary to our expectation.