Identification of hepatic encephalopathy induced proteins using by a bio-artificial liver and toxin receptor knock out hepatitis model mice

在致命性肝性脑病的治疗和诊断中，需要识别引起暴发性肝炎“快速脑水肿”的未知肝性脑病诱导物质。本研究的主要目的是鉴定脑星形胶质细胞损伤诱导血浆蛋白。一种候选致死性肝性脑病诱导蛋白，暂时命名为A蛋白，通过蛋白质组学分析得以鉴定。蛋白A对培养的星形胶质细胞具有细胞毒性。TRECK肝炎模型小鼠肝性脑病。此外，MRI DWI可观察到急性肝性脑病弥漫性星形细胞肿胀，ADC降低。下一步我们将研究A蛋白是否诱导肝性脑病的体内模型和临床病例，促进对致死性脑病的治疗。

Identification of unknown hepatic encephalopathy induced substances to cause the "rapid cerebral edema" in fulminant hepatitis is required in development of the treatment and diagnosis of fatal hepatic encephalopathy. The main purpose of this study is identification of brain astrocytes damage inducing plasma proteins. A candidate of fatal hepatic encephalopathy inducing proteins, temporary named protein A, was able to identify by proteomic analysis. Protein A induced the cytotoxicity to cultured astrocytes. Hepatic encephalopathy was induced in the TRECK hepatitis model mice. In addition, we could observe diffuse astrocyte swelling in acute hepatic encephalopathy by DWI on MRI, as a decrease in the ADC. We will study whether protein A induce hepatic encephalopathy in vivo model and clinical cases in next step, and promote the treatment for fatal encephalopathy.