Establishment of immuno-monitoring system for the infectious disease control and analysis of neutrophil dysfunction in various compromised hosts

败血症患者和控制不良的糖尿病患者中性粒细胞趋化性降低。肝硬变、系统性红斑狼疮、艾滋病、骨髓瘤、严重烧伤和老年患者的中性粒细胞杀菌能力明显低于健康对照组。脓毒症患者中性粒细胞膜抗原CD14、TLR-4表达水平较高，CD11b、CD16表达水平较低。中性粒细胞TREM-1基因的表达与脓毒症的严重程度相关。使用这些分析方法分析中性粒细胞功能，可作为敏感和可靠的免疫监测工具，用于确定宿主抵御受感染宿主的细菌感染的能力。

Neutrophil chemotaxis was reduced in septic patients and poorly controlled diabetic patients. Neutrophil bactericidal ability in the patients with liver cirrhosis, SLE, AIDS, myeloma, severe burn and elderly persons was significantly lower than that of healthy controls. Expression levels of neutrophil membrane antigens, CD14 and TLR-4 were higher in patients with sepsis ; however, the expression levels of CD11b and CD16 were lower. The gene expression of TREM-1 in neutrophils associated with the severity of sepsis. Analysis of neutrophil functions using these assays may be available as the sensitive and reliable immuno-monitoring tools for determining the capacity of host defense against bacterial infections in compromised hosts.