Research toward establishing comprehensive biological markers of pathophysiology in children with developmental disorders and early intervention.

研究建立发育障碍儿童病理生理学的综合生物标志物和早期干预。

基本信息

批准号：
21591338
负责人：
MATSUISHI Toyojiro
金额：
$ 2.75万
依托单位：
Kurume University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2009
资助国家：
日本
起止时间：
2009 至 2011
项目状态：
已结题

项目摘要

Abstract We established an innovative comprehensive noninvasive brain function analysis including near infrared spectroscopy(NIRS), eye mark recorder, SPM-SPECT, saliva cortisol, IgA, and sleep studies including both a sleep diary and actigraphy. The subjects included childhood onset of anorexia nervosa(AN) and age-and gender-matched controls. We also compared the Strength and Difficulties Questionnnaire(SDQ) in patients with attention deficit hyperactivity disorder(ADHD) and Asperger's syndrome(AS) and also compared to the normative data of the community sample.Twelve females with childhood AN(mean age, 14.4 years old) and 13 age-matched healthy female controls participated in this study. There was no significant difference in the prefrontal blood volume increases when viewing slender and obese body types and high-calorie food. On the other hand, prefrontal activation in AN was associated with imaging attachment between mother and child. SPM study demonstrated that relative increased … More rCBF in the bilateral parietal lobe and limbic lobe including the posterior cingulate cortex was observed after weight gain in early-onset AN. A significant, positive correlation was observed between BMI and rCBF in the right thalamus, right parietal lobe, and right cerebellum. Then we studied 21 females with AN and 22 control subjects using the saliva samples. Saliva was collected at 2-hour intervals from 9 a. m. to 7 p. m. before and after inpatient treatment. Mean salivary cortisol levels at all points and mean Area Under Curve(AUC) cortisol levels in subjects with AN before therapy were significantly higher than those in controls, but returned to control levels after inpatient treatment. Higher AUC cortisol levels were associated with lower standard deviation for weight in AN. A significant, positive correlation between the AUC cortisol level and POMS subscale of"Fatigue"was apparent in the control group, but not in the AN group. Changes in HPA axis activity following treatment could therefore be used to predict prognosis and particularly in the short term Unique optic function is one of the most important signs in Asperger syndrome(AS). We measured exploratory eye movement to assess optic function in AS. 29 AS patients and 26 normal control children participated. We analyzed total eye scanning length(TESL) and responsive search score(RSS) under interpersonal responses. The total TESL in AS was not different from those in control, however, the TESL showed significant negative correlation with Autism Spectrum Screening Questionnaire score, indicating that AS with shorter TESL had more autistic symptoms. The RSS in AS was significantly lower. These characteristic exploratory eye movements were different from those in juvenile onset schizophrenia. Measuring exploratory eye movements may be a biologically useful method of detecting optic dysfunction in AS. SDQ for both parents and teachers were performed. Each 30 children, aged 6-12, with high function ASD(HFASD) and ADHD, and compatered to the control community samples(n=1, 112) were enrolled in this study. When the HFASD group and AD/HD group are merged together, total difficulties score, all subscale scores were significantly higher compared to the community group with the exception of prosocial behaviour, which was significantly low. When comparing the HFASD group to the AD/HD group, the subscales of emotional symptoms and peer problems(3.0±2.4 vs. 1.6±1.8), (5.3±2.2 vs. 3.6±2.2), in HFASD and ADHD, respectively, which was significantly different in two groups. In the teacher's ratings, all the subscales except prosocial behaviour and emotional symptoms had a significant difference between the 2 groups ; hyperactivity/inattention(6.7±2.3 vs 8.3±1.3), conduct problems(3.0±2.2 vs 4.3±2.2), and peer problems(5.0±2.1 vs 3.7±2.0). Less

摘要我们建立了创新的综合性非侵入性脑功能分析，包括近红外光谱（NIRS），Eye Mark Recorder，SPM-Spect，SPM-Spect，Saliva Cortisol，IgA，IgA和睡眠研究，包括睡眠日记和行动学。这些受试者包括厌食症的童年发作（AN）和年龄和性别匹配的对照。我们还比较了注意力缺陷多动障碍（ADHD）和阿斯伯格综合征（AS）的患者的强度和困难问卷（SDQ），并与社区样本的正常数据进行了比较。与儿童相关的女性（平均年龄，14.4岁，14.4岁）和13年年龄匹配的女性对照组参与了这项研究。观看细长和肥胖的体型和高热量食物时，前额叶血容量没有显着差异。另一方面，AN中的前额叶激活与母亲和儿童之间的成像附着有关。 SPM研究表明，相对增加……在双侧脊叶和边缘叶中，包括后扣带回皮层的RCBF在早期发作An的体重增加后观察到了。右丘脑，右顶叶和右小脑在BMI和RCBF之间观察到显着的正相关。然后，我们使用唾液样本研究了21名女性，并使用22个对照对象。从9 a开始以2小时的间隔收集唾液。 m。到7 p。 m。住院治疗前后。在治疗前治疗的受试者中，所有点和平均面积（AUC）皮质醇水平的平均唾液皮质醇水平明显高于对照组的皮质醇水平，但在住院治疗后恢复为对照水平。较高的AUC皮质醇水平与An的重量标准偏差较低有关。在对照组中，AUC皮质醇水平与“疲劳”的POMS子量表之间存在显着的正相关，但在A组中却不存在。因此，可以使用治疗后HPA轴活动的变化来预测预后，尤其是在短期内独特的光学功能是阿斯伯格综合征（AS）中最重要的迹象之一。我们测量了探索性眼动运动，以评估AS中的视觉功能。 29例患者和26名正常对照儿童参加。我们在人际反应下分析了总眼扫描长度（TESL）和响应式搜索评分（RSS）。 TESL的总TESL与控制的TESL没有差异，TESL与自闭症频谱筛选问卷分数显示出显着的负相关性，表明与较短的TESL一样，TESL具有更令人印象深刻的症状。 RSS中的AS明显较低。这些特征性的探索性眼动运动与少年发作精神分裂症不同。测量探索性眼动运动可能是一种生物学上有用的方法，用于检测AS中的视觉功能障碍。为父母和老师的SDQ均进行。本研究招募了每个30名6-12岁的儿童，具有高功能ASD（HFASD）和ADHD，并与对照社区样本相结合（n = 1，112）。当HFASD组和AD/HD组合并在一起时，总的难度得分，与社区组相比，所有子量表得分都显着更高，而亲社会行为除外，这显着低。当将HFASD组与AD/HD组进行比较时，情绪症状和同伴问题的子量表（3.0±2.4 vs. 1.6±1.8）（分别在HFASD和ADHD中为5.3±2.2 vs. 3.6±2.2），这是显着的，两组的不同之处在于。在教师的评分中，除了社会行为和情绪症状以外的所有子量表之间都有2组之间的显着差异。多动症/注意力不集中（6.7±2.3 vs 8.3±1.3），引起问题（3.0±2.2 vs 4.3±2.2）和同伴问题（5.0±2.1 vs 3.7±2.0）。较少的