identification of proteins interacting with causative molecules for hereditary nephrotic syndrome and analysis of pathogenesis of it.

点击翻译按钮获取中文摘要

We identified transport protein x bounded to podocin, important molecule for proteinuria, with immunoprecipitation and LC/MS. The double staining with endothelial marker and anti-x antibody indicated that protein x was expressed in podocyte. When protein x was suppressed with siRNA in cells, podocin expression along cell membrane was much lower than control and cell shape was changed. Overexpression of protein x improved expression of mutated podocin along cell membrane. These data indicated protein x play role in membrane trafficking of podocin.