Application of SELDI ProteinChip system in pathogenesis of pediatric fulminamt hepatic failure

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We examined about cytokine expression, infiltrating lymphocytes, protein expression in explanted liver tissue of pediatric fulminant hepatic failure(FHF). Five cytokines which were expressed higher in FHF liver tissue were identified by cytokine array(RayBio). Cytokine A which showed highest expression was localized in sinus endothelial cells, and the receptor was seen in hepatocytes and ductular cells by immunohistochemistry. Infiltrating lymphocytes were predominantly cCD3+CD8+granzymeB+T-cell and their distribution in sinus was characteristic. Using SELDI ProteinChip system, three highly expressed molecules and three lowly expressed molecules were identified by CM10 Chip, and one highly and one lowly expressed molecule were identified by Q10 Chip. Further analysis of those molecules could promise to clarify the pathogenesis of pediatric FHF.