Study on gene expression imaging in the living body using radioilabelled siRNA

在培养细胞的实验中，证实了^<99m> tc标记的siRNA与基因靶的特异性mRNA结合。此外，在尝试改进用于PET的新探针时，我们成功地优化了siRNA的^<68>Ga标记条件。此外，我们还成功地用^<62>Cu标记siRNA。有趣的是，这种放射性标记的siRNA在体内也能够获得稳定性。然而，在动物实验中，放射性标记的siRNA对肿瘤的蓄积较低。

In the experiment using a cultured cell, it was proved that the ^<99m>Tc-labelled siRNA has combined with the specific mRNA about the gene target. Moreover, when improvement of new probe for a PET was tried, we succeeded in optimization of the ^<68>Ga -labelled conditions of siRNA. Furthermore, we succeeded also in labelling siRNA by ^<62>Cu. Interestingly, This radiolabelled siRNA was also able to acquire stability in vivo. However, in the animal experiment, the accumulation to the tumor of the radiolabelled siRNA was low.