Role of MIF on the process of fracture healing and the effect of its regulation

MIF对骨折愈合过程的作用及其调节作用

• 批准号: 21591909
• 负责人: ONODERA Shin
• 金额: $ 2.83万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21591909/
• 关键词: 骨折治癒; マクロファージ遊走阻止因子; ノックアウトマウス; 構造特性; 遺伝子発現; 骨形態計測; 力学試験; 骨髄細胞; 石灰化

We investigated the role of macrophage migration inhibitory factor (MIF) on the process of fracture healing using MIF KO mice. In MIF KO mice, the mechanical properties of the fracture callus were decreased at the early stage of fracture healing, which was caused at least in part by the delay in the calcification of osteoid or the decrease in the number of osteoclasts. These were considered to be associated with decreased mRNA expression of MMP-2, MT1-MMP, Cathepsin K, and alkaline phosphatase.

我们用MIF - KO小鼠研究巨噬细胞迁移抑制因子（macrophage migration inhibitory factor， MIF）在骨折愈合过程中的作用。在MIF KO小鼠中，骨折愈合早期骨折痂的力学性能下降，至少部分原因是类骨钙化延迟或破骨细胞数量减少。这些被认为与MMP-2、MT1-MMP、组织蛋白酶K和碱性磷酸酶的mRNA表达降低有关。

项目成果

Spontaneous hyaline cartilage regeneration can be induced in an osteochondral defect created in the femoral condyle using a novel double-network hydrogel.

• DOI: 10.1186/1471-2474-12-49
• 发表时间: 2011-02-22
• 期刊: BMC musculoskeletal disorders
• 影响因子: 2.3
• 作者: Yokota M;Yasuda K;Kitamura N;Arakaki K;Onodera S;Kurokawa T;Gong JP
• 通讯作者: Gong JP

Gene expression profile of the cartilage tissue spontaneously regenerated in vivo by using a novel double-network gel: comparisons with the normal articular cartilage.

• DOI: 10.1186/1471-2474-12-213
• 发表时间: 2011-09-29
• 期刊: BMC musculoskeletal disorders
• 影响因子: 2.3
• 作者: Imabuchi R;Ohmiya Y;Kwon HJ;Onodera S;Kitamura N;Kurokawa T;Gong JP;Yasuda K
• 通讯作者: Yasuda K

Impaired fracture healing in macrophage migration inhibitory factor-deficient mice

• DOI: 10.1007/s00198-010-1385-0
• 发表时间: 2011-06-01
• 期刊: OSTEOPOROSIS INTERNATIONAL
• 影响因子: 4
• 作者: Kobayashi, T.;Onodera, S.;Yasuda, K.
• 通讯作者: Yasuda, K.

マクロファージ遊走阻止因子遺伝子の欠損が骨折治癒に与える効果

巨噬细胞迁移抑制因子基因缺失对骨折愈合的影响

• 发表时间: 2011
• 作者: 小林巧;小野寺伸;遠山晴一;近藤英司;安田和則
• 通讯作者: 安田和則

DNA-vaccination against macrophage migration inhibitory factor improves atopic dermatitis in murine models.

针对巨噬细胞迁移抑制因子的 DNA 疫苗可改善小鼠模型中的特应性皮炎。

• 发表时间: 2009
• 期刊: J Allergy Clin Immunol 124
• 作者: Hamasaka A;Abe R;Koyama Y;Yoshioka N;Fujita Y;Hoshina D;Sasaki M;Hirasawa T;Onodera S;Ohshima S;Leng L;Bucala R;Nishihira J;Shimizu T;Shimizu H.
• 通讯作者: Shimizu H.