Neuroprotective effects of CV-159 viewed from energy metabolism and radical scavenge capacity

CV-159是一种选择性Ca^<2+>/钙调蛋白阻滞剂和自由基清除剂。应用磷核磁共振波谱技术研究了CV-159对大鼠脑能量代谢的保护作用。将CV-159加入到灌流液中（10 nM-10μM，体外应用），或在断头前1小时（体内应用）腹膜内给予雄性Wistar大鼠（120μg/kg）。再灌注后1小时，腹膜内CV-159给药大鼠的磷酸肌酸（活细胞中ATP的能量缓冲液）恢复水平显著高于对照组。但在体外应用中，当用含有CV-159的ACSF灌注脑切片时，未观察到神经保护作用。

CV-159 is a selective blocker of Ca^<2+>/calmodulin and a free radical scavenger. Neuroprotective effects of CV-159 on energy metabolism of rat brain were investigated by phosphorous nuclear magnetic resonance spectroscopy. CV-159 was added to superfusate(10 nM-10μM, in vitro application), or was administered intraperitoneally(120μg/kg) to male Wistar rats 1 hr before decapitation(in vivo application). The recovery level of phosphocreatine, energetic buffer of ATP in living cells, 1 h after the reperfusion was significantly higher in intraperitoneally CV-159-administered rats than in the control. But with in vitro application, no neuroprotective effect was observed when brain slices were superfused with ACSF containing CV-159.