Basic research of cancer stem cell for therapeutic target in cisplatin-resistant ovarian cancer.

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Epithelial-mesenchymal-transition(EMT) is recently identified as a important step in the invasion, metastasis, and chemo-resistance of cancer. We investigated whether topotecan increase the efficacy of cisplatin in in vitro and in vivo ovarian cancer models. Topotecan significantly inhibited the cisplatin-induced Akt activation, and regulated EMT-related factors in cisplatin-resistant ovarian cancer cell line. Furthermore, G protein-coupled receptor 30 is a 7-transmembrane estrogen receptor that functions alongside traditional estrogen receptors to regulate cellular responses to estrogen. In our studies suggested that GPR30 expression is linked to EMT phenotype, aggressive proliferation, and multiple drug resistance. Immunohistochemical examination revealed that expression of both GPR30 and EGFR contributes lower survival rates in epithelial ovarian cancer.