神経芽腫におけるMYCN遺伝子を標的としたPIポリアミドの抗腫瘍効果に関する研究

PI聚酰胺靶向MYCN基因对神经母细胞瘤的抗肿瘤作用研究

• 批准号: 21592281
• 负责人: OHASHI Kensuke
• 金额: $ 3.08万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21592281/
• 关键词: MYCN遺伝子; PIポリアミド; 発現抑制; 小児外科; 小児固形腫瘍; 癌関連遺伝子; 神経芽腫; ピロールイミダゾールポリアミド

Neuroblastoma(NB) is the most common pediatric solid tumor. NB has MYCN amplified is poor outcome and down regulation of MYCN expression causes proliferation down-regulated and differentiation in NB. We designed PI polyamide targeting for SP1 and E2F binding site, which are promoter region of MYCN gene. NB cell lines, such as CHP134 cells were cultured in the PI polyamide. NB cell lines were down-regulation of proliferation and of MYCN expression, compared with NB cell lines untreated with PI polyamide. We made xenograft nude mice by using CHP134 cells. In NB xeno-graft mice, was we administrated PI polyamide into the tumor. Then the tumors growth was down-regulated, compared with non-treated group. This PI polyamide could be one of the new therapeutic agents for MYCN gene amplified neuroblastoma.

神经母细胞瘤是儿科最常见的实体瘤。Nb中MYCN扩增结果较差，MYCN表达下调可导致Nb增殖下调和分化。我们针对MYCN基因的启动子区域SP1和E2F结合部位设计了PI聚酰胺。在聚酰亚胺中培养NB细胞系，如CHP134细胞。与未经PI聚酰胺处理的NB细胞株相比，NB细胞株的增殖和MYCN表达下调。我们用CHP134细胞制作了裸鼠移植瘤。在NB异种移植小鼠中，我们将PI聚酰胺注射到肿瘤中。与未治疗组相比，肿瘤生长受到抑制。聚酰亚胺有望成为治疗MYCN基因扩增神经母细胞瘤的新药物之一。

项目成果

Mesenchymal hamartoma of the liver originating in the caudate lobe with t(11;19)(913;913.4)

起源于尾状叶的肝脏间叶性错构瘤，t(11;19)(913;913.4)

• 发表时间: 2010
• 期刊: Surgery today
• 影响因子: 2.5
• 作者: Sugito K;Sugito K;Sugito.K
• 通讯作者: Sugito.K

神経芽腫におけるNR4A3遺伝子のメチル化解析

神经母细胞瘤NR4A3基因甲基化分析

• 发表时间: 2010
• 作者: Sugito K;Yoshizawa S;Uekusa S;Kawashima H;Furuya T;Ohashi K;Inoue M;Ikeda T;Koshinaga T;Maebayashi T;Kawashima H;植草省太,杉藤公信,吉澤信輔,川島弘之,古屋武史,益子貴行,大橋研介,井上幹也,池田太郎,越永従道,永瀬浩喜;川島弘之,杉藤公信,植草省太,古屋武史,池田太郎,越永従道,永瀬浩喜
• 通讯作者: 川島弘之,杉藤公信,植草省太,古屋武史,池田太郎,越永従道,永瀬浩喜

神経芽腫,肝芽腫における5D52領域のメチル化異常の検討

神经母细胞瘤和肝母细胞瘤 5D52 区域甲基化异常的检查

• 发表时间: 2009
• 作者: Sugito K;Yoshizawa S;Uekusa S;Kawashima H;Furuya T;Ohashi K;Inoue M;Ikeda T;Koshinaga T;Maebayashi T;Kawashima H;植草省太,杉藤公信,吉澤信輔,川島弘之,古屋武史,益子貴行,大橋研介,井上幹也,池田太郎,越永従道,永瀬浩喜;川島弘之,杉藤公信,植草省太,古屋武史,池田太郎,越永従道,永瀬浩喜;植草省太,杉藤公信,吉澤信輔,川島弘之,古屋武史,益子貴行,大橋研介,井上幹也,池田太郎,越永従道,草深竹志,永瀬浩喜;川島弘之,杉藤公信,植草省太,古屋武史,池田太郎,越永従道,草深竹志,永瀬浩喜;川島弘之,杉藤公信,植草省太,古屋武史,大橋研介,池田太郎,越永従道,草深竹志,麦島秀雄,永瀬浩喜;植草省太,杉藤公信,川島弘之,吉澤信輔,古屋武史,大橋研介,池田太郎,越永従道,草深竹志,麦島秀雄,永瀬浩喜
• 通讯作者: 植草省太,杉藤公信,川島弘之,吉澤信輔,古屋武史,大橋研介,池田太郎,越永従道,草深竹志,麦島秀雄,永瀬浩喜

Application of radiofrequency ablation for giant solid pseudopapillary tumor of the pancreas

• DOI: 10.1111/j.1442-200x.2009.02999.x
• 发表时间: 2010-02-01
• 期刊: PEDIATRICS INTERNATIONAL
• 影响因子: 1.4
• 作者: Sugito, Kiminobu;Kusafuka, Takeshi;Mugishima, Hideo
• 通讯作者: Mugishima, Hideo

Usefulness of power Doppler ultrasonography and superparamagnetic iron oxide enhanced magnetic resonance imaging for diagnosis of focal nodular hyperplasia of the liver after treatment of neuroblastoma

能量多普勒超声和超顺磁氧化铁增强磁共振成像在诊断神经母细胞瘤治疗后肝脏局灶性结节性增生中的作用

• 发表时间: 2010
• 期刊: Pediatr Hematol Oncol.
• 作者: 鳥山和宏;中里公亮;亀井譲;八木俊路朗;佐藤秀吉;小野昌史;神戸未来;Sugito K;Sugito K
• 通讯作者: Sugito K