Immune-histological observations of reconstructed gingiva-like cultures using epithelial cells derived from PDL

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This investigation showed the comparison the differentiations and proliferations of the epithelial cells derived from periodontal ligaments or Hertwig's epithelial root sheath against to gingiva. The three dimensional organotypic cultures were incubated under air-liquid interface environment. The specimens showed that the multilayered epithelial structures without keratinization and the dispersing tendency of the cells in the epithelial layer were seen after 2 weeks incubation. The results showed that the reconstructed tissues were not similar to original gingiva. The continuous proper growth without scar after transplantation to immunodeficiency mice indicated the usefulness of transplantations.