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Morphological changes of nerve by nerve block

神经阻滞引起的神经形态变化

基本信息

批准号：
21592581
负责人：
JYOU Sigeharu
金额：
$ 2.66万
依托单位：
Iwate Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2009
资助国家：
日本
起止时间：
2009 至 2011
项目状态：
已结题

项目摘要

A high dose local anesthetic, 5% lidocaine, and a semi-permanent neurolytic agent, 99% alcohol, were infused into the infraorbital foramen, and the morphological change progression from nerve injury to regeneration was observed. And the normal intraneural vascularisation of the infraorbital nerve from the infraorbital foramen to the peripheral vibrissae were examined.Immediately post drug infusion, there were no significant changes seen in either the alcohol or the lidocaine groups. One day post drug infusion, both the alcohol group and lidocaine group showed near complete destruction of the myelinated nerve fibers within the fascicle. In the alcohol group, this destructed state continued for one month post-procedure, but in the lidocaine group, by the third day post-procedure, the number of destructed myelinated nerve fibers decreased by 50%. In the alcohol group, from 2 months post-procedure to 3 months post-procedure, 75% of the myelinated nerve fibers were less than 5μm in length, … More while 20% were thick myelinated nerve fibers greater than 5μm. By 6 months post-procedure, thick myelinated nerve fibers were 70%, while thin nerve fibers were 30%, which are close to values found in the control group. In the lidocaine group, from one week post-procedure to 6 months post-procedure, the percentage of myelinated nerve fibers less than 5μm gradually decreased over time, while the percentage of fibers greater than 5μm increased. Approximately 5% remained destructed. Optical microscopic observation of the cross-section of normal mouse infraorbital nerve near the infraorbital foramen revealed nerve fiber fascicles(about 150μm in diameter) covered with epineurium extending from proximal to peripheral areas, increasing in number as they branched. In the nerve fascicle surrounded by the perineurium near the infraorbital foramen, a few blood vessels were distributed. As the nerve fascicle branched and extended, the blood vessels also branched, providing one blood vessel in each nerve fascicle. There were multiple blood vessels in between the epineurium and perineurium. The blood vessels in nerve fascicles consisted of thin branches communicating with blood vessels outside of the perineurium. Nerve fascicles were surrounded by networks of blood vessels communicating with capillaries, arteriolae and venulae. Some blood vessels showed chain-like distribution in nerve fascicles. In the peripheral part of the infraorbital nerve, the blood vessel in the nerve fascicle exited before the nerve entered a vibrissa. There were no blood vessels in the nerve fascicle at the nerve terminal. Less

将大剂量局麻药5%利多卡因和半永久性神经阻滞剂99%乙醇注入眶下孔，观察神经损伤到再生的形态学变化。观察了眶下神经从眶下孔到周围触须的正常神经内血管分布，在药物输注后即刻，酒精组和利多卡因组均未见明显变化。药物输注后1天，酒精组和利多卡因组均显示出束内有髓神经纤维的几乎完全破坏。在酒精组中，这种破坏状态持续了一个月，但在利多卡因组中，到术后第三天，破坏的有髓神经纤维数量减少了50%。酒精组术后2个月至3个月，75%的有髓神经纤维长度小于5μm， ...更多信息粗的有髓神经纤维直径大于5μm者占20%。到术后6个月，厚的有髓神经纤维为70%，而薄的神经纤维为30%，这接近于对照组中发现的值。在利多卡因组中，从术后1周至术后6个月，小于5μm的有髓神经纤维的百分比随时间逐渐减少，而大于5μm的纤维的百分比增加。约5%被摧毁。对正常小鼠眶下神经眶下孔附近的横切面进行光学显微镜观察，发现神经纤维束（直径约150μm）由近端向周围延伸，并随分支的增多而增多，外膜覆盖着神经纤维束。眶下孔附近神经束膜包绕的神经束内分布有少量血管。随着神经束的分支和延伸，血管也分支，在每个神经束中提供一个血管。神经外膜和神经束膜之间有多条血管。神经束内的血管由细支组成，与神经束膜外的血管相通。神经束被血管网包围，血管网与毛细血管、小动脉和小静脉相通。部分血管在神经束内呈链状分布。在眶下神经的外周部，神经束中的血管在神经进入触须之前就已穿出。神经末梢的神经束内无血管。少