Genome-wide mapping of cis-regulatory elements conserved in vertebrates

我们进行了顺式调控的基因参与的发展和疾病在全基因组的方式，使用比较基因组学方法和有效的转基因技术在非洲爪蟾。保守的增强子在多种基因中被鉴定，包括Pax家族和Polycomb相关基因。此外，脊椎动物和文昌鱼的顺式调控元件之间的比较功能分析表明，沉默创新，而不是增强子退化，是至关重要的在脊椎动物进化过程中的parabolic表达的多样化。

We performed cis-regulatory analyses of genes involved in development and disease in a genome-wide manner, using comparative genomics approach and an efficient transgenesis technique in Xenopus. Conserved enhancers were identified in a variety of genes including Pax family and Polycomb-related genes. Furthermore, comparative functional analysis between the vertebrate and amphioxus cis-regulatory elements revealed that silencer innovation, rather than enhancer degeneration, was crucial for the diversification of paralog expression during vertebrate evolution.