Homeostasis of Plant Mineral Nutrients and Growth- Modeling of Overall Regulation

这个关于植物矿质营养动态平衡的项目包括几个不同的方面，以了解动态平衡的机制。对于了解相对有限的营养物质，我们分离出对低营养条件有适应缺陷的拟南芥新突变体，鉴定了相关基因，并对基因产物的功能进行了鉴定。发现了钙、镁、锰、铜和钼的新基因。对硼的动态平衡机理进行了深入的分析。在该项目启动之前，我们确定了几个用于摄取和转位的硼转运蛋白。在这个项目中，我们揭示了调控的新方面，包括通过核糖体-RNA复合体感知硼。我们还构建了全面了解整体调控机制的数学模型。基于该模型，我们预测了拟南芥根中硼的积累规律，并通过实验验证了预测的正确性。

This project on the mineral nutrient homeostasis in plants includes several different aspects for the understanding of mechanisms of homeostasis. For the nutrients with relatively limited understanding, we isolated novel Arabidopsis mutants defective in acclimation to low nutrient conditions, identified responsible genes and characterized the functions of the gene products. Novel genes were found for Ca, Mg, Mn, Cu and Mo. In-depth analyses of the homeostasis mechanism of boron were also conducted. Before the initiation of the project, we identified several boron transporters for uptake and translocation. In this project we revealed novel aspects of regulation including boron sensing by ribosome-RNA complexes. We also constructed mathematical model to comprehensively understand the overall regulation mechanisms. Based on the model we predicted boron accumulation pattern in the root of Arabidopsis and the prediction was confirmed by experimental examination.