Development of wavelength-dependent fluorolabel transfer method to functional domain of target protein

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Although fluorescence-based visualization has been primarily applied to investigate the behavior of target protein in a cell, there are few methods for fluorescent labeling at the interacting site. Here, a simple and unique construction of a fluorophore at the biding domain of target protein has been achieved by sequential photoreactions using a non-fluorescent ligand molecule. The labeled protein can be used for the fluorescence-based substrate binding analysis