Functionalization of microbes cultured in 2D on the newly developed optical feedback system

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We developed a new optical feedback system for the photoreactive microbes confined in a microfluidic device. By using the photophobic reaction of Euglena, we succeeded in the control of cell density in the micro-aquarium dynamically with arbitrary pattern. We also achieved neuro-computing by introducing neural network algorithm in the feedback algorithm, by which optimized solution can be obtained with dynamic transition. Further, we succeeded in artificial connection between two individual culture system with optical feedback as well as chemical sensing using chemotaxis of the microbes.