Elucidation of the activation mechanismof photoactivated adenylyl cyclase (PAC)

本研究旨在阐明光激活腺苷酸环化酶（PAC）的激活机制，从而促进该酶的生物技术应用。我们证明了Beggiatoa PAC的细菌同源物（BsPAC）在大肠杆菌中表现出轻度依赖的腺苷酸环化酶活性。通过分析BsPAC突变体的功能，我们确定了几个在光活化酶分子内信号转导中起重要作用的氨基酸残基

This research is aimed at elucidating the activation mechanism of photoactivated adenylyl cyclase (PAC), thereby promoting biotechnological application of the enzyme. We demonstrated that a bacterial homolog of PAC from Beggiatoa(BsPAC) showed light dependent activity of adenylyl cyclase in E. coli. By analyzing the functions of BsPAC mutants, we identified several amino acid residues that have important roles for intramolecular signal transduction of the photoactivated enzyme