Analysis of mechanisms underlying treatment-resistance ofhepatocelluar carcinoma in the point view of post-translationalmodification evaluated by proteomics

尽管临床对肝细胞癌（HCC）进行了根治性治疗，包括手术切除和射频消融（RFA），但据报道，年复发率为15-10%，这表明HCC可能被归类为预后最差的癌症。为了克服这一问题，本研究旨在确定治疗耐药的关键分子，并开发一种新的HCC治疗策略。在此背景下，我们对凋亡刺激下肝癌细胞的基因和蛋白表达进行了全面分析。此外，还对翻译后修饰进行了全面分析。因此，大约有30种蛋白质被缩小了范围，因为这些蛋白质的磷酸化状态（翻译后修饰之一）在细胞凋亡刺激下显着改变。这些蛋白质包括伴侣蛋白、细胞骨架相关分子。目前正在对这些蛋白进行功能分析，以确定导致hcc耐药的关键分子。

Although curative treatment, including surgical resection and radio frequency ablation (RFA), isperformed against hepatocellular carcinoma (HCC) in the clinical setting, annual recurrence rate hasbeen reported to be 15-10%, indicating that HCC might be categorized into cancers with worst prognosis.To overcome this issue, this study is aimed to identify key-molecules responsible for treatmentresistance and develop a new therapeutic strategy for HCC. In this context, we have performedcomprehensive analysis of gene and protein expression in hepatoma cells under apoptosis stimulation. Inaddition, post-translational modification is analyzed comprehensively . Consequently , around thirtyproteins are narrowed down, because phosphorylation status, one of the post-translational modifications,of these proteins is significantly changed under apoptosis stimulation. These proteins are includingchaperon proteins, cytoskeleton -related molecules. Functional analysis is currently being performed onthese proteins in order to identify key-molecules which are responsible for treatment resistance ofHCCs.