Research for the elucidation of the cardiovascular effect and application to treatment by adenosine

腺苷被认为是心脏保护的关键调节分子。然而，它一直难以证明腺苷的心血管保护机制的分子机制。为了解决这一问题，我们首先建立了高灵敏度的血浆腺苷水平的LC-MS测定系统。其次，我们通过测定血液和心肌组织样品中的腺苷水平，证明了腺苷在各种病理生理条件下的多种心血管保护机制。此外，心力衰竭患者基因表达水平改变的澄清表明腺苷相关基因表达可能调节心力衰竭的病理生理学，并且腺苷的添加是治疗心力衰竭的候选药物。第四，我们在临床研究中发现，血浆腺苷水平可以作为心力衰竭严重程度的标志。总之，我们需要关注腺苷代谢在心血管疾病的病理生理通过本研究。

Adenosine is known to be a key regulatory molecule of cardioprotection. However, it has been difficult to demonstrate the molecular mechanisms for the cardiovascular protective mechanisms of adenosine. To solve this question, we have firstly established the highly-sensitive system of the measurements of plasma adenosine level using LC-MS. Secondly, we demonstrated the multiple cardiovascular protective mechanisms of adenosine in a variety of the pathophysiological condition by the measurement of adenosine levels in the blood and myocardial tissue samples. Moreover, the clarification of the alteration in gene expression levels in patients with heart failure implied that adenosine-related gene expression might modulate the pathophysiology of heart failure, and the addition of adenosine is a candidate for the treatment of heart failure. Fourthly, we showed that the plasma adenosine levels can be a marker of the severity of heart failure in the clinical study. Taken together, we need to focus on the adenosine metabolism in the pathophysiology of cardiovascular diseases via the present study.