Analysis of cancer metastasis using circulating tumor cells purified by micro-fluidics techniques

为了研究肺癌转移的机制和寻找肺癌转移的分子靶点，我们开发了从外周血中纯化循环肿瘤细胞（CTC）的方法，并使用纳米柱技术创建了原型装置。我们还调查了CTC的遗传图谱，并选择了CTC的特征基因。EMT调控分子ZEB1和Twist将成为肺癌转移的分子靶点。

To investigate the mechanisms and the search of the molecular targets for lung cancer metastasis, we developed the methods for purifying the circulating tumor cells(CTC) from peripheral blood, and we created the prototype device using the nanopillar technique. We also investigated the genetic profiles of CTC, and selected the genes, which were characteristic for CTC. EMT regulatory molecules of ZEB1 and Twist will become molecular targets for lung cancer metastasis.