Novel approach of induction of donor specific immune tolerance: adoptive transfer of Treg cells combined with MF1 gene transfection

诱导供者特异性免疫耐受的新方法：Treg细胞过继转移结合MF1基因转染

• 批准号: 21390446
• 负责人: AZUMA Haruhito
• 金额: $ 10.98万
• 依托单位: Osaka Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21390446/
• 关键词: CD28-Superagonist; 非特異的免疫制御細胞; Treg バンク; CD34+幹細胞; ドナー特異的な免疫寛容; MF-1遺伝子導入; レンチウイルス; 超音波照射遺伝子導入法; Tregバンク; 幹細胞共培養; 免疫寛容; CD28SA

We have developed a novel approach of highly effective gene transfection technique using lenti-virus vector combined with ultrasound mediated gene transfection procedure. We have performed successful gene transfection of metron factor, which is the engineered chimeric factors containing selected functional domains of HGF and MSP, into the transplanted kidney tissue. Local production of MF-1, which may enhance the expansion of Treg cells in vivo, combined with .the adoptive transfer of Treg cells, successfully induced the donor specific immune tolerance in more than 70% of recipients in our rat kidney transplant model.

我们开发了一种利用Lenti病毒载体和超声介导的基因转染法相结合的高效基因转染法。我们成功地将含有HGF和MSP功能区的工程化嵌合因子Metron因子基因导入到移植肾组织中。在我们的大鼠肾移植模型中，局部产生的MF-1可以促进Treg细胞的体内扩增，结合Treg细胞的过继转移，成功地诱导了70%以上的受者对供者的特异性免疫耐受。

项目成果

Conversion of prostate cancer from hormone independency to dependency due to AMACR inhibition: involvement of increased AR expression and decreased IGF1 expression.

• 发表时间: 2009-07
• 期刊: Anticancer research
• 影响因子: 2
• 作者: K. Takahara;H. Azuma;Takeshi Sakamoto;S. Kiyama;T. Inamoto;N. Ibuki;T. Nishida;H. Nomi;T. Ubai;N. Segawa;Y. Katsuoka

Rapid Increase of the Serum PSA Level in Response to High-intensity Focused Ultrasound Therapy may be a Potential Indicator of Biochemical Recurrence of Low-and Intermedicate-Risk Prostate Cancer

高强度聚焦超声治疗后血清 PSA 水平快速升高可能是低度和中度风险前列腺癌生化复发的潜在指标

• 发表时间: 2011
• 期刊: Clinical Medicine Insights : Oncology
• 作者: Inamoto T;Komura K;Watsuji T;Azuma H
• 通讯作者: Azuma H

Induction of Donor-Specific Tolerance Using Superagonistic CD28 Antibody in Rat Renal Allografts: Regulatory T-Cell Expansion Before Engraftment May Be Important

• DOI: 10.1097/tp.0b013e3182007b59
• 发表时间: 2010-12-27
• 期刊: TRANSPLANTATION
• 影响因子: 6.2
• 作者: Azuma, Haruhito;Isaka, Yoshitaka;Takahara, Shiro
• 通讯作者: Takahara, Shiro

今、求められる医療の実践を目指して

旨在实践现在需要的医疗服务

• 发表时间: 2011
• 作者: Matsumoto K;Katsumata N;Saito I;Shibata T;Konishi I;Fukuda H;Kamura T.;東治人
• 通讯作者: 東治人

Successful induction of donor-specific tolerance using Super-agonistic CD28 antibody in rat renal allografts

使用超激动 CD28 抗体在大鼠同种异体肾移植物中成功诱导供体特异性耐受

• 发表时间: 2010
• 作者: Azuma;H.;Nomi;H.;Ibuki;N.;Inamoto;T.;Isaka;Y.;Takahara;S.;Katsuoka;Y.
• 通讯作者: Y.