Analysis of autophagy related protein p62 in carcinogenesis of oral cancer

自噬相关蛋白p62在口腔癌癌变过程中的分析

• 批准号: 21390530
• 负责人: YANAGAWA Toru
• 金额: $ 11.56万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21390530/
• 关键词: 臨床腫瘍学; p62; アポトーシス; STAT3

I. in vitro analysis The purpose of this study is to reveal the association of autophagy related protein p62 and apoptosis, and carcinogenesis of oral cancer.FACS analysis revealed that p62 (-/-)MEF increased resitance to apoptosis, and the similar result was obtained from siRNA experiment.Stat3 and Src phosphorylation were increased in p62 (-/-)MEF. Real time PCR revealed Bcl-2 and Bcl-xL expression were increased. Immuno-blotting analysis showed that Bcl-2, Bcl-xL, and Bax were reduced in p62 (-/-) and wild type MEF by UVB irradiation, however, no significant difference were observed between p62 (-/-) and wild type MEF. ATM mRNA expression were not different between p62 (-/-) and wild type MEF.II. in vivo analysis By UVB irradiation to skin of p62 (-/-) and wild typ mice showed that p62 (-/-) skin had less apoptotic response resulting resistance to apoptosis.III. Additional results Prx I (-/-) MEF showed reduced resistance to apoptosis. We found NASH model mouse by using p62 (-/-) and Nrf2 (-/-) mice.

I.流式细胞仪检测p62（-/-）MEF对口腔癌细胞凋亡的抵抗性，与siRNA实验结果相似;自噬相关蛋白p62（-/-）MEF中Stat 3和Src磷酸化水平升高。真实的时间PCR显示Bcl-2和Bcl-xL表达增加。免疫印迹分析显示，UVB照射后，p62（-/-）和野生型MEF中Bcl-2、Bcl-xL和Bax的表达均降低，而p62（-/-）和野生型MEF中Bcl-2、Bcl-xL和Bax的表达无明显差异。p62（-/-）与野生型MEF之间ATM mRNA表达无差异。通过UVB照射p62（-/-）和野生型小鼠皮肤的体内分析表明，p62（-/-）皮肤具有较少的凋亡反应，导致对细胞凋亡的抗性。其他结果Prx I（-/-）MEF显示对细胞凋亡的抗性降低。用p62（-/-）和Nrf 2（-/-）小鼠建立NASH模型。

项目成果

マクロファージの抗酸化ストレス応答-転写因子Nrf2と誘導タンパク質の役割

巨噬细胞的抗氧化应激反应-转录因子Nrf2和诱导蛋白的作用

• 发表时间: 2010
• 作者: 石井哲郎;ほか
• 通讯作者: ほか

Deficiency of sequestosome 1 accelerates neointimal hyperplasia and carotid artery UK-Japan

缺乏Sequestosome 1会加速新内膜增生和颈动脉英国-日本

• 发表时间: 2012
• 作者: Sakai S;Warabi E;Katayanagi S;Yanagawa T;Ishii T
• 通讯作者: Ishii T

非アルコール性脂肪性肝炎および肝腫瘍自然発症モデルとしてのp62 : Nrf2遺伝子二重欠損マウスおよび該マウスを用いた方法

p62作为非酒精性脂肪性肝炎和自发性肝肿瘤发展的模型：Nrf2基因双缺陷小鼠和使用该小鼠的方法

• 发表时间: 2012

Deficiency of sequestosome 1 accelerrates neointimal hyperplasia and carotidartery remodeling

Sequestosome 1的缺乏会加速新内膜增生和颈动脉重塑

• 发表时间: 2012
• 作者: Taira;M.;Kagiya;T.;Sasaki;M. and Kimura;S.;Satoshi Sakai
• 通讯作者: Satoshi Sakai

Peroxiredoxin I plays a protective role against cisplatin cytotoxicity through mitogen activated kinase signals.

• DOI: 10.1016/j.oraloncology.2009.07.002
• 发表时间: 2009-12
• 期刊: Oral oncology
• 影响因子: 4.8
• 作者: Dongmei Ma;E. Warabi;T. Yanagawa;Shintaro Kimura;Harumi Harada;K. Yamagata;T. Ishii