Analysis of cleft palate signal by using CASK knockout mouse

CASK基因敲除小鼠腭裂信号分析

• 批准号: 23659935
• 负责人: YANAGAWA Toru
• 金额: $ 2.33万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23659935/
• 关键词: 口腔外科学一般; CASK; 口蓋裂; ノックアウトマウス

We hypothesize that CASK signal affects cleft palate formation because CASK knock out mouse has cleft palate. To clarify this signal we identified the candidate molecules that associate with CASK by using Q-TOF LC/MS. We selected Neurexin as a signal transduction molecule, then adherent domain and interaction between molecules was analyzed. In addition CASK flox/flox mouse was analyzed and estimated the role of CASK in cleft palate formation. On the other hand we developed a new method which evaluate morphological similarity to use mouse facial deformity.

我们假设CASK信号影响腭裂的形成，因为CASK敲除小鼠有腭裂。为了澄清这一信号，我们使用Q-TOF LC/MS鉴定了与CASK相关的候选分子。我们选择Neurexin作为信号转导分子，分析了分子间的粘附结构域和相互作用。此外，对CASK flox/flox小鼠进行分析和估计CASK在腭裂形成中的作用。另一方面，我们开发了一种以小鼠面部畸形为对象的形态学相似性评价方法。

项目成果

Presurgical nasoalveolar molding orthopedic treatment improves the outcome of primary cheiloplasty of unilateral complete cleft lip and palate, as assessed by naris morphology and cleft gap.

根据鼻孔形态和裂隙评估，术前鼻牙槽成型矫形治疗可改善单侧完全性唇腭裂初次唇整形术的结果。

• DOI: 10.1097/scs.0b013e31825196dc
• 发表时间: 2012
• 期刊: Journal of Craniofacial Surgery
• 影响因子: 0.9
• 作者: Toru Yanagawa;Shizuo Nakane;et.al.
• 通讯作者: et.al.

A novel method for evaluating postsurgical results of unilateral cleft lip and palate with the use of Hausdorff distance: presurgical orthopedic treatment improves nasal symmetry after primary cheiloplasty

• DOI: 10.1016/j.oooo.2012.01.042
• 发表时间: 2012-12-01
• 期刊: ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY
• 影响因子: 2.9
• 作者: Karube, Rei;Sasaki, Hiroyoshi;Bukawa, Hiroki
• 通讯作者: Bukawa, Hiroki