Bone regeneration procedure with tongue muscle-derived stem cell

采用微珠磁分离系统从Jcl-ICR小鼠舌肌中分离得到舌肌源性Sca-1阳性细胞（tdsc）。体外培养的tdsc分化成成骨细胞系表现为成骨细胞因子上调和茜素红或Von Kossa阳性矿化基质的形成。在β- tcp明胶海绵中观察到骨形成。这些数据表明，tdsc保留了其成骨分化潜力，并可能用于骨再生医学。

Tongue muscle-derived Sca-1 positive cells(TDSCs) were isolated from Jcl-ICR mice tongue muscle by magnetic cell separation system with microbeads. TDSCs differentiated to osteoblast lineage demonstrated by up-regulation of osteoblastic makers and formation of mineralized matrix with alizarin red or Von Kossa positive in vitro. The bone formation was observed in the gelatin sponges ofβ-TCP. These data suggest that TDSCs retain its osteogenic differentiation potential, and may be useful for regenerative medicine of bone.