Usefulness of alginate membrane added to osteoconductivity as GBR membrane

我们研制了一种新型的可生物吸收的海藻酸膜，直接应用于骨缺损的表面，证实了它在体内起到了屏障膜的作用。本研究的目的是确定用于生产海藻酸膜的海藻酸钠和氯化钙的合适浓度。随着海藻酸钠浓度的增加，海藻酸膜的厚度增加。然而，膜的吸收延迟了，残留的膜阻止了骨的形成。此外，骨缺损处被填充的海藻酸钠可能会推迟新骨的形成。然后，我们使用现成的海藻酸膜检测骨形成。结果：皮侧骨缺损处海藻酸盐膜破裂，结缔组织侵入。另一方面，从肌肉侧原有的皮质骨边缘可观察到新的骨形成。然而，…此外，新骨形成时间的缩短是不能接受的。因此，这种现成的海藻酸膜的强度和传统的可吸收膜一样弱。我们在海藻酸膜中加入了骨传导性，以缩短骨再生周期：海藻酸钠加磷酸盐制成海藻酸膜。在动物实验中，含有磷酸盐的海藻酸膜与不含磷酸盐的海藻酸膜相比，骨缺损处的骨再生周期并没有缩短。相反，使用含磷酸盐的海藻酸膜的手术野频繁发生炎症反应，进而了解到磷酸盐是引起炎症的物质，因此，海藻酸钠和氯化钙的合适浓度分别为3.0%和1%，以使海藻酸膜起到可生物吸收的屏障作用。然而，在海藻酸膜中添加骨传导性的实验并未成功。较少

We developed a new bioabsorbable alginate membrane which is applied directly to the surface of the bone defect, confirmed that it works as a barrier membrane in vivo. The purpose of the present study was to determine the appropriate concentration of sodium alginate and calcium chloride employed in the production of an alginate membrane. The thickness of alginate membranes increased with elevated sodium alginate concentration. The absorption of membrane, however, was delayed and the remained membrane prevented the bone formation.Moreover, there is a possibility that sodium alginate, which the bone defect is filled with, delays the new bone formation. Then, we examined the bone formation using the ready-made alginate membrane. The result was that a ready-made alginate membrane was broken and ingress of the connective tissue was observed for the bone defect at skin side. On the other hand, new bone formation was observed from the edge of preexisting cortical bone at muscle side. However, … More the shortening in the period of the new bone formation could not be accepted. Consequently, this ready-made alginate membrane was weak strength as well as conventional resorbable membrane.We added osteoconductivity to alginate membrane to shorten the period of bone regeneration : alginate membrane was made using sodium alginate including phosphate. In animal experiment, however, the period of bone regeneration in bone defect using alginate membrane including phosphate was not shortened compared with that using alginate membrane without phosphate. Conversely, the operation field using alginate membrane including phosphate occurred frequently inflammatory reaction, and then we understood that phosphate was affected as a material causing the inflammation.As a result, the appropriate concentration of sodium alginate and calcium chloride was each 3.0 % and 1 % so that alginate membrane works as a bioabsorbable barrier membrane. However, the experiment of adding osteoconductivity to alginate membrane did not success. Less