Usefulness of alginate membrane added to osteoconductivity as GBR membrane

添加骨传导性的藻酸盐膜作为 GBR 膜的有用性

• 批准号: 11470436
• 负责人: UEYAMA Yoshiya
• 金额: $ 9.41万
• 依托单位: Tottori University (2000-2001)Okayama University (1999)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11470436/
• 关键词: GBR membrane; alginate membrane; osteoconductivity; bone regeneration; thickness of membrane; リン酸含有アルジネート膜; 間接法; 膜の強度; 膜の吸収

We developed a new bioabsorbable alginate membrane which is applied directly to the surface of the bone defect, confirmed that it works as a barrier membrane in vivo. The purpose of the present study was to determine the appropriate concentration of sodium alginate and calcium chloride employed in the production of an alginate membrane. The thickness of alginate membranes increased with elevated sodium alginate concentration. The absorption of membrane, however, was delayed and the remained membrane prevented the bone formation.Moreover, there is a possibility that sodium alginate, which the bone defect is filled with, delays the new bone formation. Then, we examined the bone formation using the ready-made alginate membrane. The result was that a ready-made alginate membrane was broken and ingress of the connective tissue was observed for the bone defect at skin side. On the other hand, new bone formation was observed from the edge of preexisting cortical bone at muscle side. However, … More the shortening in the period of the new bone formation could not be accepted. Consequently, this ready-made alginate membrane was weak strength as well as conventional resorbable membrane.We added osteoconductivity to alginate membrane to shorten the period of bone regeneration : alginate membrane was made using sodium alginate including phosphate. In animal experiment, however, the period of bone regeneration in bone defect using alginate membrane including phosphate was not shortened compared with that using alginate membrane without phosphate. Conversely, the operation field using alginate membrane including phosphate occurred frequently inflammatory reaction, and then we understood that phosphate was affected as a material causing the inflammation.As a result, the appropriate concentration of sodium alginate and calcium chloride was each 3.0 % and 1 % so that alginate membrane works as a bioabsorbable barrier membrane. However, the experiment of adding osteoconductivity to alginate membrane did not success. Less

我们研制了一种新型的可吸收性藻酸盐膜，将其直接应用于骨缺损表面，证实其在体内起屏障膜的作用。本研究的目的是确定用于生产海藻酸盐膜的海藻酸钠和氯化钙的适当浓度。海藻酸钠膜的厚度随着海藻酸钠浓度的增加而增加。然而，膜的吸收被延迟，残留的膜阻碍了骨形成。此外，填充骨缺损的海藻酸钠可能延迟了新骨的形成。然后，我们使用现成的藻酸盐膜检查骨形成。结果是现成的藻酸盐膜破裂，皮肤侧骨缺损观察到结缔组织侵入。另一方面，在肌肉侧的既存皮质骨边缘观察到新骨形成。然而，在这方面， ...更多信息 不能接受新骨形成期的缩短。因此，这种现成的海藻酸盐膜强度和传统的可吸收膜一样弱，我们在海藻酸盐膜中加入了骨传导性，以缩短骨再生的时间：海藻酸盐膜是由含磷酸盐的海藻酸钠制成的。但在动物实验中，含磷酸盐的藻酸盐膜修复骨缺损的时间并没有比不含磷酸盐的藻酸盐膜缩短。相反，使用含磷酸盐的藻酸盐膜的手术区域经常发生炎症反应，然后我们了解到磷酸盐作为引起炎症的材料受到影响。结果，藻酸钠和氯化钙的合适浓度分别为3.0%和1%，使得藻酸盐膜作为生物可吸收屏障膜发挥作用。然而，将骨传导性添加到藻酸盐膜的实验没有成功。少

项目成果

Ueyama Y, Ishikawa K, Mano T, Koyama T, et al.: "Usefulness as guied bone regeneration membrane of the alginate membrane"Biomaterials. (2002)

Ueyama Y、Ishikawa K、Mano T、Koyama T 等人：“藻酸盐膜作为引导骨再生膜的用途”生物材料。

Ueyama Y, Ishikawa K, Mano T, Koyama T, et al.: "Initial tissue response to anti-washout apatite cement in the rat palatal region : comparison with conventional apatine cement"J Biomed Mater Res. 55. 652-660 (2002)

Ueyama Y、Ishikawa K、Mano T、Koyama T 等人：“大鼠腭区对抗冲刷磷灰石水泥的初始组织反应：与传统磷灰石水泥的比较”J Biomed Mater Res。

Ueyama Y, Ishikawa K, Mano T, Koyama T, et al.: "Initial tissue response to anti-washout apatite cement in the rat palatal region : comparison with conventional apatite cement"Journal of Biomedical Materials Research. 55. 652-660 (2001)

Ueyama Y、Ishikawa K、Mano T、Koyama T 等人：“大鼠腭区对抗冲刷磷灰石水泥的初始组织反应：与传统磷灰石水泥的比较”生物医学材料研究杂志。

Ishikawa K, Ueyama Y, Mano T, Koyama T, et al: "Self-setting barrier membrane for guided tissue regeneration method : Initial evaluation of alginate membrane made with sodium alginate and calcium chloride aqueous solution"Journal of Biomedical Materials R

Ishikawa K, Ueyama Y, Mano T, Koyama T, et al：“用于引导组织再生方法的自凝固屏障膜：用海藻酸钠和氯化钙水溶液制成的海藻酸盐膜的初步评价”Journal of Biomedical Materials R

Ueyama Y, Ishikawa K, Mano T, Koyama T, et al.: "Usefulness as guided bone regeneration membrane of the alginate membrane"Biomaterials. (2002)

Ueyama Y、Ishikawa K、Mano T、Koyama T 等人：“藻酸盐膜作为引导骨再生膜的有用性”生物材料。