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EXAMINATION OF TELOMERASE ACTIVITY AND TUMOR SUPPRESSOR GENE ON HUMAN ORAL CANCERS

人类口腔癌端粒酶活性和抑癌基因的检测

基本信息

批准号：
09672048
负责人：
UEYAMA Yoshiya
金额：
$ 2.37万
依托单位：
OKAYAMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09672048/
关键词：
telomerase oral cancer cancer chemotherapy cisplatin resistant cell

项目摘要

The purpose of this study is to examine what influence anticancer drugs will exercise on telomerase. It has been reported that cancer cells can report cell division endlessly since they express telomerase which makes telomera lengthen. On the other hand, cisplatin, which is the most frequent anticancer drug in every region, was used in this study. in clinical specimens from oral malignant tumor patients, the high. value of telomerase activity were shown in approximate 80% of them. In the in vitro experiment which was used the uterine cancer cell line, A431 and it's resistant cell lines (C-1, C-2), the value of telomerase activity in the resistant cell lines showed higher than that in the original cell line, And in the in vivo experiment which cisplatin was administered to these cell lines tumors made in the back of nude mouse, Group A431 was recognized the decrease of tumors volume. However, cisplatin was ineffective against C-1 and C-2 cell lines for group C-1and C-2 increased tumors volume. On the other hand, inhibition of telomerase activity was observed in group A431, but no telomerase inhibition in group C-1 and C-2. The same studies performed in in vivo and in vitro gave similar results. The result was that there existed a definite correlation between cisplatin sensitivity and telomerase activity, In this study, it is suggested that telomerase activity is useful in the index of cisplatin sensitivity.

本研究的目的是研究抗癌药物对端粒酶的影响。据报道，癌细胞可以不断地报告细胞分裂，因为它们表达端粒酶，使端粒延长。另一方面，本研究中使用的顺铂是各地区最常用的抗癌药物。在口腔恶性肿瘤患者的临床标本中，高。端粒酶活性值显示约80%。用子宫癌细胞系A431及其耐药细胞系（C-1、C-2）进行体外实验，发现耐药细胞系端粒酶活性值高于原细胞系，体内实验中给予顺铂治疗裸鼠背部肿瘤的细胞系，发现A431组肿瘤体积减小。然而，顺铂对C-1和C-2细胞系无效，C-1和C-2组肿瘤体积增加。另一方面，A431组对端粒酶活性有抑制作用，而C-1和C-2组端粒酶活性无抑制作用。在体内和体外进行的相同研究得出了相似的结果。结果表明顺铂敏感性与端粒酶活性之间存在一定的相关性，本研究提示端粒酶活性可作为顺铂敏感性指标。