EXAMINATION OF TELOMERASE ACTIVITY AND TUMOR SUPPRESSOR GENE ON HUMAN ORAL CANCERS

本研究旨在探讨抗癌药物对端粒酶的影响。据报道，癌细胞可以无休止地报告细胞分裂，因为它们表达使端粒延长的端粒酶。另一方面，本研究中使用了顺铂，这是每个地区最常用的抗癌药物。在口腔恶性肿瘤患者的临床标本中，端粒酶活性阳性率约80%。在体外实验中，采用子宫癌细胞系A431及其耐药细胞系（C-1，C-2），耐药细胞系的端粒酶活性值高于原始细胞系。A431组肿瘤体积缩小。而顺铂对C-1和C-2细胞系无效，因为C-1和C-2组肿瘤体积增加。另一方面，A431组中观察到端粒酶活性抑制，而C-1和C-2组中未观察到端粒酶抑制。在体内和体外进行的相同研究得出了相似的结果。结果表明，端粒酶活性与顺铂敏感性之间存在一定的相关性，端粒酶活性可作为顺铂敏感性的指标。

The purpose of this study is to examine what influence anticancer drugs will exercise on telomerase. It has been reported that cancer cells can report cell division endlessly since they express telomerase which makes telomera lengthen. On the other hand, cisplatin, which is the most frequent anticancer drug in every region, was used in this study. in clinical specimens from oral malignant tumor patients, the high. value of telomerase activity were shown in approximate 80% of them. In the in vitro experiment which was used the uterine cancer cell line, A431 and it's resistant cell lines (C-1, C-2), the value of telomerase activity in the resistant cell lines showed higher than that in the original cell line, And in the in vivo experiment which cisplatin was administered to these cell lines tumors made in the back of nude mouse, Group A431 was recognized the decrease of tumors volume. However, cisplatin was ineffective against C-1 and C-2 cell lines for group C-1and C-2 increased tumors volume. On the other hand, inhibition of telomerase activity was observed in group A431, but no telomerase inhibition in group C-1 and C-2. The same studies performed in in vivo and in vitro gave similar results. The result was that there existed a definite correlation between cisplatin sensitivity and telomerase activity, In this study, it is suggested that telomerase activity is useful in the index of cisplatin sensitivity.