Linear tetrapyrroles and phytochrome-like sensors in signal transduction of the opportunistic pathogen Pseudomonas aeruginosa

线性四吡咯和光敏色素样传感器在机会性病原体铜绿假单胞菌信号转导中的作用

• 批准号: 5385900
• 负责人: Professorin Dr. Nicole Frankenberg-Dinkel
• 金额: --
• 依托单位: Fachgebiet Mikrobiologie
• 依托单位国家: 德国
• 项目类别: Independent Junior Research Groups
• 财政年份: 2002
• 资助国家: 德国
• 起止时间: 2001-12-31 至 2006-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5385900?language=en
• 关键词: Linear; tetrapyrroles; phytochrome; like; sensors

This proposal focuses on the investigation of the role of linear tetrapyrroles (bilins) in bacterial signal transduction. For many pathogenic bacteria like Pseudomonas aeruginosa heme is an essential source of iron. After uptake, the heme molecule is degraded by heme oxygenases to yield iron, carbon monoxide and biliverdin. The heme oxygenase PigA is only induced under iron limiting conditions, the gene for a second putative heme oxygenase, bphO of this non-photosynthetic bacterium occurs in an operon with the gene bphP encoding a bacterial phytochrome. The major objective of this proposal is the functional characterization of the two heme oxygenases and the formed biliverdins in combination with the bacterial phytochrome-like sensor BphP. The role of BphP in bilin binding and sensing, and its contribution to iron- and heme regulated processes in the overall regulatory network of P. aeruginosa will be determined using a combination of genetic, biochemical and modern methods of proteomics and genomics. Long term consequences for biofilm formation and infection are of general interest.

本研究主要探讨线性四吡咯（bilins）在细菌信号转导中的作用。对于许多致病菌，如铜绿假单胞菌，血红素是铁的重要来源。摄取后，血红素分子被血红素加氧酶降解，产生铁、一氧化碳和胆绿素。血红素加氧酶PigA仅在铁限制条件下被诱导，该非光合细菌的第二种推定的血红素加氧酶bphO的基因与编码细菌光敏色素的基因bphP一起出现在操纵子中。本提案的主要目的是结合细菌光敏色素样传感器BphP的两个血红素加氧酶和形成的胆绿素的功能表征。BphP在胆色素结合和传感中的作用，以及其对铜绿假单胞菌整体调控网络中铁和血红素调控过程的贡献将使用遗传学，生物化学和现代蛋白质组学和基因组学方法的组合来确定。生物膜形成和感染的长期后果受到普遍关注。