Study of anaphase initiation mechanism using monaphase.

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Sensor for separase activity was improved and it became sensitive enough to monitor separase activity during the metaphase to anaphase transition. I found that separase was tightly repressed much of metaphase and it was activated just prior to the onset of anaphase. As monaphase cells were synchronous, the cells were useful for biochemical analysis. Using these cells for biochemical assay, the mechanism of the tight regulation was revealed.