Identification of mammalian ovulation enzymes using in vitro ovulation system and elucidation of ovulation mechanism.

本研究的目的是确定在排卵过程中导致卵泡壁降解的排卵酶，并阐明排卵过程中卵泡壁降解的分子机制。还试图通过优化缓冲体系和改变添加的补充剂来改善培养条件。结果发现，排卵前后有两种MMPs的表达发生了变化。然而，有人认为它们与排卵无关。排卵前MT2-MMPs活性升高，提示其参与排卵。

The aim of the present study were to identify the mouse ovulation enzymes responsible for degradation of the follicle wall during ovulation, and to elucidate the molecular mechanism of degradation of the follicle wall during ovulation. It was also attempted to improve the culture condition by optimizing the buffer system and by changing the supplements added. As results, it was found that two MMPs were changed their expression between before and after ovulation. However, it was suggested that they were not involved in ovulation. Active form of MT2-MMP was found to be increased before ovulation, implying that it was involved in ovulation.