Identification of mammalian ovulation enzymes using in vitro ovulation system and elucidation of ovulation mechanism.

利用体外排卵系统鉴定哺乳动物排卵酶并阐明排卵机制。

• 批准号: 21770067
• 负责人: OGIWARA Katsueki
• 金额: $ 3万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21770067/
• 关键词: 卵巣; 排卵; プロテアーゼ; マウス

The aim of the present study were to identify the mouse ovulation enzymes responsible for degradation of the follicle wall during ovulation, and to elucidate the molecular mechanism of degradation of the follicle wall during ovulation. It was also attempted to improve the culture condition by optimizing the buffer system and by changing the supplements added. As results, it was found that two MMPs were changed their expression between before and after ovulation. However, it was suggested that they were not involved in ovulation. Active form of MT2-MMP was found to be increased before ovulation, implying that it was involved in ovulation.

本研究的目的是确定在排卵过程中导致卵泡壁降解的排卵酶，并阐明排卵过程中卵泡壁降解的分子机制。还试图通过优化缓冲体系和改变添加的补充剂来改善培养条件。结果发现，排卵前后有两种MMPs的表达发生了变化。然而，有人认为它们与排卵无关。排卵前MT2-MMPs活性升高，提示其参与排卵。

项目成果

メダカ排卵時におけるプロスタグランジン及びその受容体の解析

青鳉排卵过程中前列腺素及其受体的分析

• 发表时间: 2009
• 作者: 藤森千加;萩原茜;荻原克益;高橋孝行
• 通讯作者: 高橋孝行

メダカ排卵実行酵素MT2-MMPの発現誘導機構

青鳉排卵执行酶MT2-MMP的表达诱导机制

• 发表时间: 2008
• 作者: 荻原克益;高橋孝行
• 通讯作者: 高橋孝行

PA/プラスミン系による基底膜の分解がメダカ排卵には必要不可欠である

PA/纤溶酶系统对基底膜的降解对于青鳉排卵至关重要。

• 发表时间: 2010
• 作者: 荻原克益;高橋孝行
• 通讯作者: 高橋孝行

Expression of cyclooxygenase-2 and prostaglandin receptor EP4b mRNA in the ovary of the medaka fish, Oryzias latipes: Possible involvement in ovulation

• DOI: 10.1016/j.mce.2010.09.015
• 发表时间: 2011-01-30
• 期刊: MOLECULAR AND CELLULAR ENDOCRINOLOGY
• 影响因子: 4.1
• 作者: Fujimori, Chika;Ogiwara, Katsueki;Takahashi, Takayuki
• 通讯作者: Takahashi, Takayuki

メダカ排卵酵素MT2-MMPの排卵誘導メカニズム-排卵に関与する新規チロシンキナーゼの探索と同定

青鳉排卵酶MT2-MMP的排卵诱导机制-一种参与排卵的新型酪氨酸激酶的搜索和鉴定

• 发表时间: 2010
• 作者: 荻原克益;高橋孝行
• 通讯作者: 高橋孝行