Elucidation of the endocrine regulatory mechanism common in vertebrate ovulation.

阐明脊椎动物排卵中常见的内分泌调节机制。

• 批准号: 23687009
• 负责人: OGIWARA Katsueki
• 金额: $ 17.97万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (A)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23687009/
• 关键词: ovulation; endocrinology; LH; progesterone; ovary; 排卵; メダカ; 黄体形成ホルモン; プロゲステロン; プロテアーゼ; 内分泌; 卵巣

The aim of the present study is to elucidate the endocrine regulatory mechanism of ovulation in the medaka. The study disclosed a mechanism for inducing nuclear progestin receptor (nPR), which was the essential transcription factor for induction of ovulation-associated genes. It was revealed that nPR was involved in the induction of MT2-MMP and PAI-1, both which played an important role on ovulation. An expression pattern of the synthesizing enzymes for a maturation inducing hormone (17a, 20b-DHP), a ligand of nPR, was elucidated. Based on the results, we propose the novel model of the endocrine regulatory mechanism for inducing the ovulation-related enzymes responsible for degradation of follicle layer during ovulation.

本研究旨在阐明青竹排卵的内分泌调节机制。这项研究揭示了一种诱导核孕激素受体(NPR)的机制，核孕激素受体是诱导排卵相关基因的关键转录因子。结果表明，NPR参与了MT2-MMPs和PAI-1的诱导，两者在排卵过程中起着重要作用。阐明了NPR的配体--成熟诱导激素(17a，20b-DHP)合成酶的表达模式。基于这一结果，我们提出了内分泌调节机制的新模型，以诱导排卵相关酶在排卵过程中负责卵泡层的降解。

项目成果

メダカ排卵関連遺伝子の発現誘導に関与する核内プロゲスチン受容体nPRの発現誘導機構

核孕激素受体nPR参与诱导青鳉排卵相关基因表达的表达诱导机制

• 发表时间: 2013
• 作者: 松八重一代;中島謙一、林誠一、長坂徹也;荻原克益;Atsuki Matsubara;Masakazu Iwai;松八重一代;荻原克益
• 通讯作者: 荻原克益

nPRによるメダカ排卵酵素MT2-MMPの誘導機構の解明

nPR阐明青鳉排卵酶MT2-MMP的诱导机制

• 发表时间: 2013
• 作者: 松八重一代;中島謙一、林誠一、長坂徹也;荻原克益
• 通讯作者: 荻原克益

A proposed model for molecular mechanism of follicle rupture during medaka ovulation

青鳉排卵期间卵泡破裂分子机制的提出模型

• 发表时间: 2012
• 作者: Reuter;M. A.; Hudson;C.; van Schaik;A.; Heiskanen;K.; Meskers;C.; Hagelüken;C. (Contributors : Kazuyo Matsubae;Shinsuke Murakami;Kenichi Nakajima);荻原克益
• 通讯作者: 荻原克益

New evidence for the involvement of prostaglandin receptor EP4b in ovulation of the medaka, Oryzias latipes

• DOI: 10.1016/j.mce.2012.05.013
• 发表时间: 2012-10-15
• 期刊: MOLECULAR AND CELLULAR ENDOCRINOLOGY
• 影响因子: 4.1
• 作者: Fujimori, Chika;Ogiwara, Katsueki;Takahashi, Takayuki
• 通讯作者: Takahashi, Takayuki

メダカ排卵酵素MT2-MMPの発現誘導に関与するnPRの誘導機構-新規誘導経路の発見

nPR参与诱导青鳉排卵酶MT2-MMP表达的诱导机制——发现新的诱导途径

• 发表时间: 2011
• 作者: 荻原克益;高橋孝行
• 通讯作者: 高橋孝行