Analysis of OsMADS87 in the control of cellularization and reproductive barrier in the endosperm obtained from rice interspecific crosses

当进行种间或倍间杂交时，胚乳经常表现出发育缺陷。利用杂交胚乳RNA转录组分析发现拟南芥PHERES1基因家族的水稻同源物失调。在对OsMADS87表达的详细分析中，我们发现OsMADS87表达水平与细胞化时间相关。这些结果提示，phes1基因家族的调控系统可能参与了早期胚乳发育的合子后屏障。对过表达或RNAi构建的转基因植物也进行了分析，但对其胚乳发育没有显著影响。可能有几个OsMADS87家族基因也参与了早期胚乳发育的控制。

Endosperm often exhibits developmental defects when interspecific or interploidy crosses are conducted. Analysis of transcriptome using RNA from hybrid endosperm identified deregulation of rice homologs of Arabidopsis PHERES1 gene family. In the detailed analysis of OsMADS87 expression, it was found that OsMADS87 expression levels were correlated with the timing of cellularization. These results suggest that possible involvement of regulatory systems of PHERES1 gene family in post-zygotic barrier of early endosperm development. Analysis of transgenic plants with overexpression or RNAi constructs were also carried out, however, not significant effect was observed in their endosperm development. It might be possible that several OsMADS87 family genes are also involved in the control of early endosperm development.