Analysis of OsMADS87 in the control of cellularization and reproductive barrier in the endosperm obtained from rice interspecific crosses

OsMADS87 对水稻种间杂交胚乳细胞化和生殖屏障控制的分析

• 批准号: 21780003
• 负责人: ISHIKAWA Ryo
• 金额: $ 2.91万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21780003/
• 关键词: 栽培イネ; 野生イネ; 胚乳; 生殖隔離; イネ

Endosperm often exhibits developmental defects when interspecific or interploidy crosses are conducted. Analysis of transcriptome using RNA from hybrid endosperm identified deregulation of rice homologs of Arabidopsis PHERES1 gene family. In the detailed analysis of OsMADS87 expression, it was found that OsMADS87 expression levels were correlated with the timing of cellularization. These results suggest that possible involvement of regulatory systems of PHERES1 gene family in post-zygotic barrier of early endosperm development. Analysis of transgenic plants with overexpression or RNAi constructs were also carried out, however, not significant effect was observed in their endosperm development. It might be possible that several OsMADS87 family genes are also involved in the control of early endosperm development.

当进行种间或倍性间杂交时，胚乳经常表现出发育缺陷。利用杂交胚乳的RNA进行转录组分析，鉴定了拟南芥PHERES 1基因家族的水稻同源物的失调。在OsMADS87表达的详细分析中，发现OsMADS87表达水平与细胞化的时间相关。这些结果表明，PHERES 1基因家族的调控系统可能参与了胚乳发育的合子后屏障。对转基因植株的胚乳发育进行了分析，发现转基因植株的胚乳发育没有受到明显的影响。OsMADS87家族的一些基因可能也参与了胚乳早期发育的调控。

项目成果

Estimation of outcrossing rate of Asian wild rice, Oryza rufipogon, under field condition.

田间条件下亚洲野生稻（Oryza rufipogon）异交率的估计。

• 发表时间: 2010
• 作者: Phan P.D.T.;Kageyama H.;Ishikawa R.;Ishii T.
• 通讯作者: Ishii T.

QTL analysis for spikelet opening time using backcross inbred lines between Oryza sativa Nipponbare and O. rufipogon

利用日本晴和野生稻回交自交系进行小穗开放时间的 QTL 分析

• 发表时间: 2010
• 作者: Pham Thien Thanh;Phan Dang Thai Phuong;Ryo Ishikawa;Takashige Ishii
• 通讯作者: Takashige Ishii

QTL analysis for flowering time using backcross population between Oryza sativa Nipponbare and O. rufipogon

• DOI: 10.1266/ggs.85.273
• 发表时间: 2010-08-01
• 期刊: GENES & GENETIC SYSTEMS
• 影响因子: 1.1
• 作者: Pham Thien Thanh;Phuong Dang Thai Phan;Ishii, Takashige
• 通讯作者: Ishii, Takashige

QTL analysis for useful agronomic characters using backcross inbred lines between Oryza rufipogon and O.sativa Japonica Nipponbare.

使用野生稻和日本晴回交自交系对有用农艺性状进行 QTL 分析。

• 发表时间: 2009
• 作者: Thanh;P.T.;Ishikawa R.;Mori N.;Ishii T.
• 通讯作者: Ishii T.

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