Studies on the solid surface-binding mechanism of an intrinsically disordered protein and development of specific biological/inorganic interfaces
Studies on the solid surface-binding mechanism of an intrinsically disordered protein and development of specific biological/inorganic interfaces
批准号:
21780096
负责人:
IKEDA Takeshi
金额:
$2.91万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Young Scientists (B)
财政年份:
2009
资助国家:
日本
项目状态:
已结题
起止时间:
2009 至 2010
中文摘要
二氧化硅结合蛋白“Si标签”属于本质无序(ID)蛋白家族,其作为水溶液中快速波动结构的动态集合体存在。实验和理论研究表明,在硅标签的ID区域一致的行动,以强烈结合到二氧化硅表面。由于在其延伸的无序区域中相对大量的残基,Si-标签应该与二氧化硅表面的大面积相互作用。我们的研究表明,柔性ID蛋白质具有将生物分子连接到无机材料的巨大潜力。
英文摘要
The silica-binding protein "Si-tag" belongs to a family of intrinsically disordered (ID) proteins that exist as dynamic ensembles of rapidly fluctuating structures in aqueous solution. Experimental and theoretical studies have shown that the ID regions in Si-tag act in unison to bind strongly to silica surfaces. Si-tag should interact with a large area of the silica surface due to the relatively large number of residues in its extended, disordered regions. Our study suggests that flexible ID proteins have tremendous potential for connecting biomolecules to inorganic materials.
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DOI:
10.1016/j.pep.2011.01.012
发表时间:
2011-06
期刊:
Protein expression and purification
影响因子:
1.6
作者:
[Takeshi Ikeda;K. Motomura;Y. Agou;Takenori Ishida;R. Hirota;A. Kuroda]
通讯作者:
Takeshi Ikeda;K. Motomura;Y. Agou;Takenori Ishida;R. Hirota;A. Kuroda
シリコン結合タンパク質を用いたシリコン基板上へのタンパク質固定化
使用硅结合蛋白将蛋白质固定在硅基底上
DOI:
--
发表时间:
2010
期刊:
影响因子:
--
作者:
[池田丈, 本村圭, 阿部陽介, 雨宮嘉照, 福山正隆, 横山新, 黒田章夫]
通讯作者:
黒田章夫
DOI:
10.1016/j.pep.2009.12.009
发表时间:
2010-05-01
期刊:
PROTEIN EXPRESSION AND PURIFICATION
影响因子:
1.6
作者:
[Ikeda, Takeshi, Ninomiya, Ken-ichi, Kuroda, Akio]
通讯作者:
Kuroda, Akio
シリカ結合タンパク質Si-tag中の天然変性領域の機能解析
二氧化硅结合蛋白 Si-tag 中自然变性区域的功能分析
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[池田丈, 本村圭, 吾郷友哉, 廣田隆一, 黒田章夫]
通讯作者:
黒田章夫
シリカ結合タンパク質Si-tagを利用した変性条件下でのアフィニティー精製法の開発
使用二氧化硅结合蛋白 Si-tag 开发变性条件下的亲和纯化方法
DOI:
--
发表时间:
2010
期刊:
影响因子:
--
作者:
[本村圭, 池田丈, 吾郷友哉, 廣田隆一, 黒田章夫]
通讯作者:
黒田章夫
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