Proteomic analysis of defense factors on fish skin surface

鱼皮是鱼类重要的防御器官之一。为了进一步了解鱼类皮肤防御机制，对基因组序列数据已公开的模式鱼类河豚的皮肤粘液进行蛋白质组学分析。在分析的10多个蛋白质中，获得了4个蛋白质的内部氨基酸序列，虽然它们没有出现防御分子。用杀鲑气单胞菌和迟缓爱德华氏菌浸泡河豚，并与对照河豚比较蛋白质斑点的模式。因此，A.杀鲑性非致病性细菌对河豚鱼的形态无影响。另一方面，用E.能感染河豚的迟缓症。这些发现表明，河豚可以改变防御机制，根据细菌的物种。

Fish skin is one of the important defense organ. For further understanding of fish skin defense mechanism, proteomic analysis of skin mucus of fugu, a model fish species whose genome sequence data has been made publicly available. Among analyzed more than 10 proteins, internal amino acid sequences of 4 proteins were obtained, although they did not appear defense molecules. Furthermore, fugu were immersed with either Aeromonus salmonicida or Edwardsiella tarda and pattern of protein spots were compared with control fugu. As a result, A. salmonicida, non-pathogenic bacterium for fugu, did not affect the pattern. On the other hand, expressions of three proteins with molecular masses of 15, 44 and 75 kDa were induced when fugu were stimulated with E. tarda that can infect to fugu. These findings suggest that fugu can alter the defense mechanism in accordance with bacterial species.