Proteomic analysis of defense factors on fish skin surface

鱼皮是重要的防御器官之一。为了进一步了解鱼类的皮肤防御机制，我们对河豚的皮肤粘液进行了蛋白质组学分析，河豚是一种已经公开的模式鱼类，其基因组序列数据。在分析的10多个蛋白质中，虽然没有出现防御分子，但获得了4个蛋白质的内部氨基酸序列。同时，将河豚分别与沙门氏气单胞菌和迟缓爱德华菌分别浸泡，并与对照河豚进行蛋白斑分布的比较。结果，沙门氏菌，一种河豚的非致病性细菌，对这种模式没有影响。另一方面，用感染河豚的迟缓e菌刺激河豚时，诱导了3种分子量分别为15、44和75 kDa的蛋白的表达。这些发现表明河豚可以根据细菌种类改变防御机制。

Fish skin is one of the important defense organ. For further understanding of fish skin defense mechanism, proteomic analysis of skin mucus of fugu, a model fish species whose genome sequence data has been made publicly available. Among analyzed more than 10 proteins, internal amino acid sequences of 4 proteins were obtained, although they did not appear defense molecules. Furthermore, fugu were immersed with either Aeromonus salmonicida or Edwardsiella tarda and pattern of protein spots were compared with control fugu. As a result, A. salmonicida, non-pathogenic bacterium for fugu, did not affect the pattern. On the other hand, expressions of three proteins with molecular masses of 15, 44 and 75 kDa were induced when fugu were stimulated with E. tarda that can infect to fugu. These findings suggest that fugu can alter the defense mechanism in accordance with bacterial species.